Bacteria with phosphorus (P) solubilization potential are considered vital in promoting bioavailability of phosphorus in soil. The present study was conducted to isolate and study the variation of phosphate solubilizing potential of bacteria isolated from virgin and agricultural soils. Total 30 isolates from virgin soil and 4 isolates from agricultural soil which retained their activity on repeated subculturing were selected. Among the isolates, there was insignificant difference in the total bacterial count from virgin and agricultural soils, however, a significant difference was found in the phosphate solubilizing bacteria (PSB) count and their P solubiling potential. Soil organic matter and available P content were correlated with PSB count. The mean solubilization index (SI) was higher from the isolates from virgin soils. Equal distribution method was employed to categorize the bacterial isolates into low, medium, and high P solubilizers which depicted H ≥ 89.44 and L ≤ 68. Among all the isolates, 23.53% were high P solubilizers (P-89.44–110.88 µg/ml), 55.88% were medium P solubilizers (P- 68–89.44 µg/ml), and 20.58% isolates produced low soluble P (46.56–68 µg/ml). Analysis of the data showed that all the isolates categorized under high P solubilizers belonged to the virgin soil. The isolates were characterized based upon biochemical characterization and belonged to Pseudomonadaceae, Enterobacteriaceae, Bacillaceae, Paenibacillaceae, Micrococcaceae, Burkholderiaceae, Flavobacteriaceae, and Streptococcaceae families. 16 sRNA sequencing of the two isolates showing maximum P solubilization were characterized as Enterobacter hormaechi . However, they differ appreciably in their P solubilization at different temperatures.

中文翻译：

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旁遮普邦原始土壤和农业土壤中磷酸盐溶解细菌的变化

具有磷 (P) 增溶潜力的细菌被认为对促进土壤中磷的生物利用度至关重要。本研究旨在分离和研究从原始土壤和农业土壤中分离的细菌溶解磷酸盐的潜力的变化。总共选择了 30 个来自原始土壤的分离物和 4 个来自农业土壤的分离物，这些分离物在重复传代培养时保留了它们的活性。在分离株中，来自原始土壤和农业土壤的细菌总数差异不显着，但在磷酸盐溶解细菌（PSB）计数和磷溶解潜力方面发现显着差异。土壤有机质和速效磷含量与 PSB 计数相关。来自原始土壤的分离株的平均溶解指数 (SI) 更高。采用均等分布法将细菌分离株分为低、中、高 P 增溶剂，H ≥ 89.44 和 L ≤ 68。在所有分离株中，23.53% 为高 P 增溶剂（P-89.44–110.88 µg/ml） , 55.88% 是中 P 增溶剂 (P-68–89.44 µg/ml)，20.58% 分离株产生低可溶性 P (46.56–68 µg/ml)。数据分析表明，所有归类为高 P 增溶剂的分离株都属于原始土壤。分离物基于生化特征进行表征，属于假单胞菌科、肠杆菌科、芽孢杆菌科、类芽孢杆菌科、微球菌科、伯克霍尔德菌科、黄杆菌科和链球菌科。显示出最大 P 溶解度的两个分离株的 16 sRNA 测序被表征为 Enterobacter hormaechi。然而，