Nanoparticle-enhanced coatings of bone implants are a promising method to facilitate sustainable wound healing, leading to an increase in patient well-being. This article describes the in vitro characterization of osteoblast cells interacting with polyelectrolyte multilayers, which contain detonation nanodiamonds (NDs), as a novel class of carbon-based coating material, which presents a unique combination of photoluminescence and drug-binding properties. The cationic polyelectrolyte, namely polydiallyldimethylammonium chloride (PDDA), has been used to immobilize NDs on silica glass. The height of ND-PDDA multilayers varies from a minimum of 10 nm for one bilayer to a maximum of 90 nm for five bilayers of NDs and PDDA. Human fetal osteoblasts (hFOBs) cultured on ND-PDDA multilayers show a large number of focal adhesions, which were studied via quantitative fluorescence imaging analysis. The influence of the surface roughness on the filopodia formation was assessed via scan...

中文翻译：

---

含有爆炸纳米金刚石的聚电解质多层膜上成骨细胞的体外表征。

骨植入物的纳米粒子增强涂层是一种有前途的方法，可促进可持续伤口愈合，从而提高患者的幸福感。本文描述了成骨细胞与聚电解质多层相互作用的体外表征，其中包含爆炸纳米金刚石 (ND)，作为一种新型的碳基涂层材料，具有光致发光和药物结合特性的独特组合。阳离子聚电解质，即聚二烯丙基二甲基氯化铵 (PDDA)，已被用于将 NDs 固定在石英玻璃上。ND-PDDA 多层膜的高度从一个双层的最小 10 nm 到 ND 和 PDDA 的五个双层的最大 90 nm 不等。在 ND-PDDA 多层膜上培养的人胎儿成骨细胞 (hFOBs) 显示出大量粘着斑，通过定量荧光成像分析对其进行研究。通过扫描评估表面粗糙度对丝状伪足形成的影响。