Abstract

Under a single 4 hour inhalation exposure to aerosol containing an aqueous suspension of calcium oxide nanoparticles (CaO) in an actual concentration of 6.68 mg/m³, the accumulation of calcium increased in the lungs (by 1.52 times), in the brain (by 1.55 times) and in the liver (by 1.25 times) relative to the control, while exposure to a microdispersed analog in a concentration of 6.37 mg/m³ resulted in an increase in the indicator only in the brain (by 1.27 times). At the same time, the calcium concentration in the brain of animals in the experimental group is higher than in the comparison group (by 1.22 times). After exposure to CaO nano- and microparticles a subarachnoid hemorrhage in the brain tissues was found. The development of pathomorphological changes in the form of lymphoid tissue hyperplasia, eosinophilia, acute arterial plethora, and hemorrhagic lung infarcts, as well as the hydropic and hyaline-drop dystrophy of parenchymal liver tissue was observed only after exposure to CaO nanoparticles. The identified features of the bioaccumulation and pathomorphological changes under inhalation exposure to CaO nanoparticles demonstrate that the studied nanomaterial has a higher level of toxicity in comparison to its microdispersed analog.

中文翻译：

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一次性吸入暴露下氧化钙纳米颗粒的毒性研究与评估

摘要

在一次吸入中，暴露于实际浓度为6.68 mg / m ^3的氧化钙纳米颗粒（CaO）水悬浮液的气溶胶中，暴露4小时后，肺中钙的积累增加了（1.52倍）。 1.55倍）和相对于对照在肝脏中（1.25倍），同时暴露于浓度为6.37 mg / m ³的微分散类似物导致该指标仅在大脑中增加（增加了1.27倍）。同时，实验组动物大脑中的钙浓度比对照组高1.22倍。暴露于CaO纳米和微粒后，在脑组织中发现了蛛网膜下腔出血。仅在暴露于CaO纳米粒子后，才观察到淋巴组织增生，嗜酸性粒细胞增多，急性动脉性胸膜炎和出血性肺梗死以及实质性肝组织的水样和透明质营养不良等形式的病理形态学变化。