Phosphorus (P) is the second most crucial nutrient for plant growth after nitrogen. However, its highly reactive nature causes formation of insoluble derivatives and limits uptake by the plant roots. The wide spread applications of P based chemical fertilizers cause detrimental effects on soil fertility, agricultural product quality and environment. In this regard, phosphate-solubilizing microorganisms (PSMs) stand out as the most remarkable and promising tools for the development of safer and sustainable technologies. As a result of this, many bacterial and fungal species with significant phosphate-solubilizing activity have been discovered by using the conventional screening methods. However, the growing need for the discovery of new strains of PSMs necessitates the replacement or support to the time-consuming conventional methods with techniques that are more sensitive, reliable, reproducible and less time consuming. In this context, molecular tools and techniques provide novel approaches for microbial phosphate solubilization research. Hence, in this review information on the molecular approaches for the PSMs research is provided and its importance explained. The review also discusses the genes related to phosphate solubilizing mechanisms and molecular tools for screening these genes.

中文翻译：

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农业视角下微生物溶磷研究中分子工具的整合

磷 (P) 是仅次于氮的植物生长的第二重要营养元素。然而，其高反应性会导致形成不溶性衍生物并限制植物根部的吸收。磷基化肥的广泛应用对土壤肥力、农产品质量和环境造成不利影响。在这方面，磷酸盐溶解微生物 (PSM) 作为开发更安全和可持续技术的最显着和最有前途的工具而脱颖而出。因此，通过使用常规筛选方法，已经发现了许多具有显着磷酸盐溶解活性的细菌和真菌物种。然而，对发现新的 PSM 菌株的需求日益增长，因此需要用更灵敏、更可靠、可重复且耗时更少的技术来替代或支持耗时的传统方法。在此背景下，分子工具和技术为微生物磷酸盐溶解研究提供了新方法。因此，在这篇综述中，提供了有关 PSM 研究的分子方法的信息，并解释了其重要性。该综述还讨论了与磷酸盐溶解机制相关的基因和筛选这些基因的分子工具。在这篇综述中，提供了有关 PSM 研究的分子方法的信息，并解释了其重要性。该综述还讨论了与磷酸盐溶解机制相关的基因和筛选这些基因的分子工具。在这篇综述中，提供了有关 PSM 研究的分子方法的信息，并解释了其重要性。该综述还讨论了与磷酸盐溶解机制相关的基因和筛选这些基因的分子工具。