The adsorption behavior of AgNPs-DNA nanocomposites on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dimyristoyl-sn-glycero-3-phosphoserine (DMPS) lipid monolayers has been investigated by analyzing adsorption dynamic process, surface pressure-area isotherms (π-A), excess mean area (\({\Delta \mathrm{A}}_{\mathrm{e}\mathrm{x}\mathrm{c}}\)), excess Gibbs free energy (\(\Delta {\mathrm{G}}_{\mathrm{e}\mathrm{x}}\)) and the atomic force microscopy (AFM). The nanocomposites were prepared by sliver nanoparticles (AgNPs) and deoxyribonucleic acid (DNA) via electrostatic attraction. The result of ∆π/π and π-A isotherms indicated that the binding of AgNPs-DNA tends to adsorb on DMPC while the concentration of DNA exceeds 0.4 μg/mL. Excess mean area and thermodynamic analysis of excess Gibbs energy demonstrated that AgNPs-DNA enlarged the interval between DMPC and DMPS molecules, whose phenomena might be the electrostatic repulsion of each AgNPs-DNA molecule. AFM observation showed that the adsorption behavior of AgNPs-DNA on DMPS monolayers had a saturation point, while DMPC was not. Taken together, this work might provide a novel idea and useful basis for the development of metal nanoparticles-based biological materials.

通过分析吸附作用，研究了AgNPs-DNA纳米复合物在1,2-二肉豆蔻酰基-sn-甘油-3-磷酸胆碱（DMPC）和1,2-二肉豆蔻酰基-sn-甘油-3-磷酸丝氨酸（DMPS）脂质单分子膜上的吸附行为。动态过程，表面压力面积等温线（π-A），平均面积超额（\（{\ Delta \ mathrm {A}} _ {\ mathrm {e} \ mathrm {x} \ mathrm {c}} \\））） ，多余的吉布斯自由能（\（\ Delta {\ mathrm {G}} _ {\ mathrm {e} \ mathrm {x}} \））和原子力显微镜（AFM）。纳米复合材料是通过静电吸引通过银纳米颗粒（AgNPs）和脱氧核糖核酸（DNA）制备的。Δπ/π和π-A等温线的结果表明，当DNA浓度超过0.4μg/ mL时，AgNPs-DNA的结合倾向于吸附在DMPC上。过量的吉布斯能量的平均面积过大和热力学分析表明，AgNPs-DNA扩大了DMPC和DMPS分子之间的间隔，其现象可能是每个AgNPs-DNA分子的静电排斥。AFM观察表明，AgNPs-DNA在DMPS单层上的吸附行为具有饱和点，而DMPC没有。两者合计，这项工作可能为基于金属纳米粒子的生物材料的发展提供一个新颖的想法和有用的基础。