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Studied on the interaction between Ag-DNA nanocomposites and lipids monolayers

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Abstract

The adsorption behavior of AgNPs-DNA nanocomposites on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dimyristoyl-sn-glycero-3-phosphoserine (DMPS) lipid monolayers has been investigated by analyzing adsorption dynamic process, surface pressure-area isotherms (π-A), excess mean area (\({\Delta \mathrm{A}}_{\mathrm{e}\mathrm{x}\mathrm{c}}\)), excess Gibbs free energy (\(\Delta {\mathrm{G}}_{\mathrm{e}\mathrm{x}}\)) and the atomic force microscopy (AFM). The nanocomposites were prepared by sliver nanoparticles (AgNPs) and deoxyribonucleic acid (DNA) via electrostatic attraction. The result of ∆π/π and π-A isotherms indicated that the binding of AgNPs-DNA tends to adsorb on DMPC while the concentration of DNA exceeds 0.4 μg/mL. Excess mean area and thermodynamic analysis of excess Gibbs energy demonstrated that AgNPs-DNA enlarged the interval between DMPC and DMPS molecules, whose phenomena might be the electrostatic repulsion of each AgNPs-DNA molecule. AFM observation showed that the adsorption behavior of AgNPs-DNA on DMPS monolayers had a saturation point, while DMPC was not. Taken together, this work might provide a novel idea and useful basis for the development of metal nanoparticles-based biological materials.

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References

  • Allon, N., Saxena, A., Chambers, C., et al.: A new liposome-based gene delivery system targeting lung epithelial cells using endothelin antagonist. J. Control Release 160(2), 217–224 (2012)

    CAS  PubMed  Google Scholar 

  • Carnevalle, R.J., Luciano, C.: Lipids mediating the interaction of metronidazole with cell membrane models at the air-water interface. Colloids Surf. B 171, 377–382 (2018)

    Google Scholar 

  • Du, T., Lu, J., Liu, L.Z., et al.: Antiviral activity of graphene oxide−silver nanocomposites by preventing viral entry and activation of the antiviral innate. Appl. Bio Mater. 1, 1286–1293 (2018)

    CAS  Google Scholar 

  • Elderdfi, M., Sikorski, A.F.: Langmuir-monolayer methodologies for characterizing protein-lipid interactions. Chem. Phys. Lipid. 212, 61–72 (2018)

    CAS  Google Scholar 

  • Elzoghby, A.O., Samy, W.M., Elgindy, N.A.: Protein-based nanocarriers as promising drug and gene delivery systems. J. Control Release 161(1), 38–49 (2012)

    CAS  PubMed  Google Scholar 

  • Fang, B.Y., An, J., Liu, B., et al.: Hybridization induced fluorescence enhanced DNA-Ag nanocluster/aptamer probe for detection of prostate-specific antigen. Colloids Surf. B 175, 358–364 (2019)

    CAS  Google Scholar 

  • Galdamez, A., Serrano, A., Santana, G., et al.: DNA probe functionalization on different morphologies of ZnO/Au nanowire for bio-sensing applications. Mater. Lett. 235, 250–253 (2019)

    CAS  Google Scholar 

  • Gonçalves, G.E.G., Morais, T.R., Lago, J.H.G., et al.: Incorporation of polygodial in Langmuir films of selected lipids. Thin Solid Films 669, 19–28 (2019)

    Google Scholar 

  • Kodama, M., Shibata, O., Nakamura, S., et al.: A monolayer study on three binary mixed systems of dipalmitoyl phosphatidyl choline with cholesterol, cholestanol and stigmasterol. Colloids Surf. B 33(3–4), 211–226 (2004)

    CAS  Google Scholar 

  • Kurosaki, T., Higuchi, N., Kawakami, S., et al.: Self-assemble gene delivery system for molecular targeting using nucleic acid aptamer. Gene 491(2), 205–209 (2012)

    CAS  PubMed  Google Scholar 

  • Liu, Q., Li, N., Wang, M., et al.: A label-free fluorescent biosensor for the detection of protein kinase activity based on gold nanoclusters/graphene oxide hybrid materials. Anal. Chim. Acta 1013, 71–78 (2018a)

    CAS  PubMed  Google Scholar 

  • Liu, X.P., Chen, J.S., Mao, C.J., et al.: Enhanced photoelectrochemical DNA sensor based on TiO2/Au hybrid structure. Biosens. Bioelectron. 116, 23–29 (2018b)

    CAS  PubMed  Google Scholar 

  • Lockhart, C., Klimov, D.K.: The Alzheimer’s disease Aβ peptide binds to the anionic DMPS lipid bilayer. Biochim. Biophys. Acta 1858, 1118–1128 (2016)

    CAS  PubMed  Google Scholar 

  • Mariela, E.S.B., Maria, E.M., Miguel, V., et al.: Membrane effects of dihydropyrimidine analogues with larvicidal activity. Colloids Surf. B 150, 106–113 (2017)

    Google Scholar 

  • Medeiros, P.V.C., Gueorguiev, G.K., Stafström, S.: Bonding, charge rearrangement and interface dipoles of benzene, graphene, and PAH molecules on Au(111) and Cu(111). Carbon 81, 620–628 (2015)

    CAS  Google Scholar 

  • Mirska, D., Schirmer, K., Funari, S.S., et al.: Biophysical and biochemical properties of a binary lipid mixture for DNA transfection. Colloids Surf. B 40(1), 51–59 (2005)

    CAS  Google Scholar 

  • Nakahara, H., Hagimori, M., Mukai, T., et al.: Monolayers of a tetrazine-containing Gemini amphiphile: interplays with biomembrane lipids. Colloids Surf. B 164, 1–10 (2018)

    CAS  Google Scholar 

  • Nobre, T.M., Pavinatto, F.J., Caseli, L., et al.: Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models. Thin Solid Films 593, 158–188 (2015)

    CAS  Google Scholar 

  • Paredes, J.I., Villarrodi, S., Martínezalonso, A., et al.: Graphene oxide dispersions in organic solvents. Langmuir 24, 10560–10564 (2008)

    CAS  PubMed  Google Scholar 

  • Qu, H.J., Hao, C.C., Zhang, Z.Y., et al.: Adsorption behavior of DNA on phosphatidylcholine at the air-water interface. Mater. Sci. Eng. C 99, 505–510 (2019)

    CAS  Google Scholar 

  • Serro, A.P., Galante, R., Kozica, A., et al.: Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: liposomes and monolayers. Colloids Surf. B 116, 63–71 (2014)

    CAS  Google Scholar 

  • Silva, R.L.C.G., Silva, H.F.O., Silva, G.L.H., et al.: Lipopolysaccharides and peptidoglycans modulating the interaction of Au naparticles with cell membranes models at the air-water interface. Biophys. Chem. 238, 22–29 (2018a)

    PubMed  Google Scholar 

  • Silva, R.L.C.G., Silva, H.F.O., Silva, G.L.H., et al.: How the interaction of PVP-stabilized Ag nanoparticles with models of cellular membranes at the air-water interface is modulated by the monolayer composition. J. Colloid Interface Sci. 512, 792–800 (2018b)

    Google Scholar 

  • Souza, K.D., Perez, K.R., Durán, N., et al.: Interaction of violacein in models for cellular membranes: regulation of the interaction by the lipid composition at the air-water interface. Colloids Surf. B 160, 247–253 (2017)

    Google Scholar 

  • Srivastava, S., Nykypanchuk, D., Fukuto, M., et al.: Two-dimensional DNA-programmable assembly of nanoparticles at liquid interfaces. JACS 136, 8323–8332 (2014)

    CAS  Google Scholar 

  • Stefaniu, C., Brezesinski, G., Möhwald, H.: Langmuir monolayers as models to study processes at membrane surfaces. Adv. Colloid Interface 208, 197–213 (2014)

    CAS  Google Scholar 

  • Zhao, W., Yang, H.Y., Xu, S., et al.: Preparation of molecularly imprinted polymer/Au nanohybrids as an effective biosensing material. Colloids Surf. A 555, 95–102 (2018)

    CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (11874039), the Fundamental Research Funds for the Central Universities (GK201803021) and the National University Science and Technology Innovation Project (CX2019078).

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Correspondence to Hongjin Qu or Changchun Hao.

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Qu, H., Hao, C., Yin, Z. et al. Studied on the interaction between Ag-DNA nanocomposites and lipids monolayers. Adsorption 26, 1345–1353 (2020). https://doi.org/10.1007/s10450-020-00242-3

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  • DOI: https://doi.org/10.1007/s10450-020-00242-3

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