Accelerating wound healing is a key consideration for surgeons. The three stages of wound healing include the inflammatory response, cell proliferation and tissue repair, and much research has focused on the migration and proliferation of epidermal cells, since this is one of the most important steps in wound healing. Studies have shown that adipose mesenchymal stem cells (ADSCs) can promote wound healing by releasing exosomes, although the specific mechanism remains unclear. To clarify the role of adipose mesenchymal stem cell exosomes (ADSCs-exo), we constructed a HaCaT cells model and a mouse wound healing model to examine the effects of ADSCs-exo on wound healing. CCK8 assays and the scratch test showed that ADSCs-exo could promote the proliferation and migration of HaCaT cells. Western blotting and real-time PCR showed that ADSCs-exo upregulated the phosphorylation of AKT and the expression of HIF-1α in HaCaT cells. HIF-1α expression was reduced by inhibiting AKT phosphorylation,and the migration of HaCaT cells simultaneously slowed. These results were also confirmed in vivo. In conclusion, we confirmed that ADSCs-exo promote the proliferation and migration of HaCaT cells by regulating the activation of the AKT/HIF-1α signaling pathway, thus promoting wound healing.

中文翻译：

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脂肪间充质干细胞外泌体通过激活 AKT/HIF-1α 轴加速角质形成细胞迁移和增殖来促进伤口愈合。

加速伤口愈合是外科医生的一个关键考虑因素。伤口愈合的三个阶段包括炎症反应、细胞增殖和组织修复，许多研究都集中在表皮细胞的迁移和增殖上，因为这是伤口愈合中最重要的步骤之一。研究表明，脂肪间充质干细胞（ADSCs）可以通过释放外泌体促进伤口愈合，但具体机制尚不清楚。为了阐明脂肪间充质干细胞外泌体 (ADSCs-exo) 的作用，我们构建了 HaCaT 细胞模型和小鼠伤口愈合模型，以检测 ADSCs-exo 对伤口愈合的影响。CCK8检测和划痕试验表明ADSCs-exo可以促进HaCaT细胞的增殖和迁移。Western blotting和real-time PCR显示ADSCs-exo上调了HaCaT细胞中AKT的磷酸化和HIF-1α的表达。通过抑制AKT磷酸化降低HIF-1α的表达，同时HaCaT细胞的迁移速度减慢。这些结果也在体内得到证实。总之，我们证实ADSCs-exo通过调节AKT/HIF-1α信号通路的激活来促进HaCaT细胞的增殖和迁移，从而促进伤口愈合。