Abstract
Accelerating wound healing is a key consideration for surgeons. The three stages of wound healing include the inflammatory response, cell proliferation and tissue repair, and much research has focused on the migration and proliferation of epidermal cells, since this is one of the most important steps in wound healing. Studies have shown that adipose mesenchymal stem cells (ADSCs) can promote wound healing by releasing exosomes, although the specific mechanism remains unclear. To clarify the role of adipose mesenchymal stem cell exosomes (ADSCs-exo), we constructed a HaCaT cells model and a mouse wound healing model to examine the effects of ADSCs-exo on wound healing. CCK8 assays and the scratch test showed that ADSCs-exo could promote the proliferation and migration of HaCaT cells. Western blotting and real-time PCR showed that ADSCs-exo upregulated the phosphorylation of AKT and the expression of HIF-1α in HaCaT cells. HIF-1α expression was reduced by inhibiting AKT phosphorylation,and the migration of HaCaT cells simultaneously slowed. These results were also confirmed in vivo. In conclusion, we confirmed that ADSCs-exo promote the proliferation and migration of HaCaT cells by regulating the activation of the AKT/HIF-1α signaling pathway, thus promoting wound healing.
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02 July 2020
In the original publication of the article, the name of one of the corresponding authors was published incorrectly. The name should be 'Ke Tao' instead of 'Kao Tao'. The corrected author group is given in this Correction.
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This study was supported by National Natural Science Foundation of China (No. 81871561).
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YZ, methodology data curation, writing original draft, review and editing. FH, methodology, validation, investigation, writing review & editing, Visualization. LG, writing original draft, formal analysis. PJ, resources, investigation, validation. XKY, resources investigation and validation. ML, investigation and validation. KT, review and editing, funding acquisition. DH. Conceptualization, writing review & editing, Supervision, Project administration.
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All mice were conducted in accordance with the National Institutes of Health Guide for the care and use of laboratory animals and approved by the Biological Research Ethics Committee of the Chinese Academy of Sciences.
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10735_2020_9887_MOESM1_ESM.tif
Electronic supplementary material 1 Supplementary Figure. Scratch test in HIF-1α silenced HaCaT cells. HaCaT cells were divided into four groups: the control group, HIF-1α siRNA group, exosomes group and exosomes + HIF-1α siRNA group. A. The level of siHIF-1α in HIF-1α silenced HaCaT cells. B-C. The distance from the scratch was measured at 0, 24, and 48h in each group after stimulation and scratch. Bar = 50μm, **p<0.01, n = 6. (TIF 1480 kb)
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Zhang, Y., Han, F., Gu, L. et al. Adipose mesenchymal stem cell exosomes promote wound healing through accelerated keratinocyte migration and proliferation by activating the AKT/HIF-1α axis. J Mol Hist 51, 375–383 (2020). https://doi.org/10.1007/s10735-020-09887-4
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DOI: https://doi.org/10.1007/s10735-020-09887-4