In the biological environment, the fate of nanomaterials characterizes as critical matter, which regulates environmental effects and associated hazards for humans. Understanding of the nanoparticle’s degradation, transformations and persistence may predict these risks. Safely designed inorganic nanomaterials are being focused for therapy; yet, fundamental processing in the biological environment and physical properties have not been assessed thoroughly. In this research work, bare, polyethylene glycol and citrate-coated cobalt ferrite nanoparticles (NPs) are prepared through modified chemical coprecipitation method. Structural, elemental, magnetic and morphological analysis of synthesized samples are performed through XRD, EDX, FTIR, VSM, SEM and TEM. XRD confirms the cubic structure of CoFe₂O₄ with crystallite size 25.75 nm. SEM and TEM confirm the formation of faceted cube-like morphology. For in vivo toxicity studies, a single dose of bare and coated cobalt ferrite NPs are intraperitoneally administrated in healthy albino rats. The degradation effects are studied through optical follow-up, by introducing bare and coated NPs in lysosomal-like media where changes in behavior are linked with transformations in vivo. Transfer of degraded ions of cobalt ferrite NPs into apoferritin are also evaluated. Apoferritin studies reveal partial filling of protein with cobalt ions from cobalt ferrite NPs.

在生物环境中，纳米材料的命运表征为关键物质，可调节环境影响和对人类的危害。了解纳米粒子的降解，转化和持久性可以预测这些风险。安全设计的无机纳米材料正被用于治疗。但是，尚未对生物环境和物理性质中的基本加工进行彻底评估。在这项研究工作中，通过改进的化学共沉淀方法制备了裸露的聚乙二醇和柠檬酸盐包覆的钴铁氧体纳米粒子（NP）。通过XRD，EDX，FTIR，VSM，SEM和TEM对合成样品进行结构，元素，磁性和形态分析。XRD证实了CoFe ₂ O ₄的立方结构微晶尺寸为25.75 nm。SEM和TEM证实了多面立方体状形态的形成。对于体内毒性研究，在健康的白化病大鼠中腹膜内施用单剂量的裸露和包衣的钴铁氧体NP。通过光学跟踪研究降解效果，方法是在溶酶体样培养基中引入裸露和包被的NP，其中行为的改变与体内的转化有关。还评估了铁氧体钴NP降解离子向载铁蛋白的转移。载铁蛋白的研究表明，钴铁氧体NP中的钴离子会部分填充蛋白质。