Abstract
In the biological environment, the fate of nanomaterials characterizes as critical matter, which regulates environmental effects and associated hazards for humans. Understanding of the nanoparticle’s degradation, transformations and persistence may predict these risks. Safely designed inorganic nanomaterials are being focused for therapy; yet, fundamental processing in the biological environment and physical properties have not been assessed thoroughly. In this research work, bare, polyethylene glycol and citrate-coated cobalt ferrite nanoparticles (NPs) are prepared through modified chemical coprecipitation method. Structural, elemental, magnetic and morphological analysis of synthesized samples are performed through XRD, EDX, FTIR, VSM, SEM and TEM. XRD confirms the cubic structure of CoFe2O4 with crystallite size 25.75 nm. SEM and TEM confirm the formation of faceted cube-like morphology. For in vivo toxicity studies, a single dose of bare and coated cobalt ferrite NPs are intraperitoneally administrated in healthy albino rats. The degradation effects are studied through optical follow-up, by introducing bare and coated NPs in lysosomal-like media where changes in behavior are linked with transformations in vivo. Transfer of degraded ions of cobalt ferrite NPs into apoferritin are also evaluated. Apoferritin studies reveal partial filling of protein with cobalt ions from cobalt ferrite NPs.
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References
Abakumov MA et al (2018) Toxicity of iron oxide nanoparticles: size and coating effects. J Biochem Mol Toxicol 32:e22225
Abudayyak M, Gurkaynak TA, Özhan G (2017) vitro toxicological assessment of cobalt ferrite nanoparticles in several mammalian cell types. Biol Trace Elem Res 175:458–465
Ahmad F, Zhou Y (2017) Pitfalls and challenges in nanotoxicology: a case of cobalt ferrite (CoFe2O4) nanocomposites. Chem Res Toxicol 30:492–507
Ajroudi L, Mliki N, Bessais L, Madigou V, Villain S, Leroux C (2014) Magnetic, electric and thermal properties of cobalt ferrite nanoparticles. Mater Res Bull 59:49–58
Akhtar S et al (2018) Toxicity of PEG-coated CoFe 2 O 4 nanoparticles with treatment effect of curcumin. Nanoscale Res Lett 13:52
Akhtar S et al (2019) A comparative study of the toxicity of polyethylene glycol-coated cobalt ferrite nanospheres and nanoparticles. Nanoscale Res Lett 14:386
Al Lehyani S, Hassan R, Alharbi A, Alomayri T, Alamri H (2017) Magnetic hyperthermia using cobalt ferrite nanoparticles: the influence of particle size. Int J Adv Technol 8:1000196–1000201
Baldi G, Bonacchi D, Innocenti C, Lorenzi G, Sangregorio C (2007) Cobalt ferrite nanoparticles: the control of the particle size and surface state and their effects on magnetic properties. J Magn Magn Mater 311:10–16
Behrens S, Appel I (2016) Magnetic nanocomposites. Curr Opin Biotechnol 39:89–96
Bregar VB, Lojk J, Šuštar V, Veranič P, Pavlin M (2013) Visualization of internalization of functionalized cobalt ferrite nanoparticles and their intracellular fate. Int J Nanomed 8:919
Chasteen ND, Harrison PM (1999) Mineralization in ferritin: an efficient means of iron storage. J Struct Biol 126:182–194
Cho W-S et al (2011) Progressive severe lung injury by zinc oxide nanoparticles; the role of Zn 2+ dissolution inside lysosomes. Part Fibre Toxicol 8:27
Colognato R, Bonelli A, Bonacchi D, Baldi G, Migliore L (2007) Analysis of cobalt ferrite nanoparticles induced genotoxicity on human peripheral lymphocytes: comparison of size and organic grafting-dependent effects. Nanotoxicology 1:301–308
Darwish MS, Kim H, Lee H, Ryu C, Lee JY, Yoon J (2019) Synthesis of magnetic ferrite nanoparticles with high hyperthermia performance via a controlled co-precipitation method. Nanomaterials 9:1176
L Ding H, X Zhang Y, H Li G (2014) Recent research progress on magnetic nanocomposites with silica shell structures preparation and nanotheranostic applications. Recent Pat Nanotechnol 8:117–128
El-Sayed AA, Hussein MM, Soliman AH (2018) Naringenin and hesperidin ameliorate iron oxide nanoparticles toxicity in rat liver. Arab J Med Sci 1(1):26–30
Ezzaier H, Marins J, Claudet C, Hemery G, Sandre O, Kuzhir P (2018) Kinetics of aggregation and magnetic separation of multicore iron oxide nanoparticles: effect of the grafted layer thickness. Nanomaterials 8:623
Fan XA, Guan J, Cao X, Wang W, Mou F (2010) Low-temperature synthesis, magnetic and microwave electromagnetic properties of substoichiometric spinel cobalt ferrite octahedra. Eur J Inorg Chem 3:419–426
Feng Q, Liu Y, Huang J, Chen K, Huang J, Xiao K (2018) Uptake, distribution, clearance, and toxicity of iron oxide nanoparticles with different sizes and coatings. Sci Rep 8:2082
Gözüak F, Köseoğlu Y, Baykal A, Kavas H (2009) Synthesis and characterization of CoxZn1− xFe2O4 magnetic nanoparticles via a PEG-assisted route. J Magn Magn Mater 321:2170–2177
Gul I, Maqsood A (2008) Structural, magnetic and electrical properties of cobalt ferrites prepared by the sol–gel route. J Alloys Compd 465:227–231
Hankiewicz J et al (2019) Nano-sized ferrite particles for magnetic resonance imaging thermometry. J Magn Magn Mater 469:550–557
Horev-Azaria L et al (2013) Predictive toxicology of cobalt ferrite nanoparticles: comparative in-vitro study of different cellular models using methods of knowledge discovery from data. Part Fibre Toxicol 10:32
Jain TK, Reddy MK, Morales MA, Leslie-Pelecky DL, Labhasetwar V (2008) Biodistribution, clearance, and biocompatibility of iron oxide magnetic nanoparticles in rats. Mol Pharm 5:316–327
Jaiswal MK, Gogoi M, Sarma HD, Banerjee R, Bahadur D (2014) Biocompatibility, biodistribution and efficacy of magnetic nanohydrogels in inhibiting growth of tumors in experimental mice models. Biomater Sci 2:370–380
Javed Y et al (2014) Biodegradation mechanisms of iron oxide monocrystalline nanoflowers and tunable shield effect of gold coating. Small 10:3325–3337. https://doi.org/10.1002/smll.201400281
Kapilevich L et al (2010) Effect of nanodisperse ferrite cobalt (CoFe2O4) particles on contractile reactions in guinea pigs airways. Bull Exp Biol Med 149:70
Kidosaki T, Takase S, Shimizu Y (2012) Electrodeposited cobalt-iron alloy thin-film for potentiometric hydrogen phosphate-ion sensor. J Sens Technol 2:95
Klostergaard J, Seeney CE (2012) Magnetic nanovectors for drug delivery. Maturitas 73:33–44
Kolosnjaj-Tabi J et al (2015) The one year fate of iron oxide coated gold nanoparticles in mice Acs. NANO 9:7925–7939. https://doi.org/10.1021/acsnano.5b00042
Krishna R, Titus E, Chandra S, Bardhan NK, Krishna R, Bahadur D, Gracio J (2012) Fabrication of a glucose biosensor based on citric acid assisted cobalt ferrite magnetic nanoparticles. J Nanosci Nanotechnol 12:6631–6638
Kumar V, Rana A, Yadav M, Pant R (2008) Size-induced effect on nano-crystalline CoFe2O4. J Magn Magn Mater 320:1729–1734
Kumari M, Rajak S, Singh SP, Murty US, Mahboob M, Grover P, Rahman MF (2013) Biochemical alterations induced by acute oral doses of iron oxide nanoparticles in Wistar rats. Drug Chem Toxicol 36:296–305
Lartigue L et al (2013) Biodegradation of iron oxide nanocubes: high-resolution in situ monitoring Acs. NANO 7:3939–3952
Lee DS, Kim S (2012) Gene expression profiles for genotoxic effects of silica-free and silica-coated cobalt ferrite nanoparticles. J Nucl Med 53:106–112
Li L, Mak K, Leung C, Chan K, Chan W, Zhong W, Pong P (2013) Effect of synthesis conditions on the properties of citric-acid coated iron oxide nanoparticles. Microelectron Eng 110:329–334
Liu N et al (2013) Degradation of aqueous synthesized CdTe/ZnS quantum dots in mice: differential blood kinetics and biodistribution of cadmium and tellurium. Part Fibre Toxicol 10:37
López-Ortega A, Lottini E, Fernández CdJ, Sangregorio C (2015) Exploring the magnetic properties of cobalt-ferrite nanoparticles for the development of a rare-earth-free permanent magnet. Chem Mater 27:4048–4056. https://doi.org/10.1021/acs.chemmater.5b01034
Margabandhu M, Sendhilnathan S, Senthilkumar S, Gajalakshmi D (2016) Investigation of structural, morphological, magnetic properties and biomedical applications of Cu2+ substituted uncoated cobalt ferrite nanoparticles. Braz Arch Biol Technol. https://doi.org/10.1590/1678-4324-2016161046
Markova-Deneva I (2010) Infrared spectroscopy investigation of metallic nanoparticles based on copper, cobalt, and nickel synthesized through borohydride reduction method. J Univ Chem Technol Metall 45:351–378
Marmorato P et al (2011) Cellular distribution and degradation of cobalt ferrite nanoparticles in Balb/3T3 mouse fibroblasts. Toxicol Lett 207:128–136
Millot N, Le Gallet S, Aymes D, Bernard F, Grin Y (2007) Spark plasma sintering of cobalt ferrite nanopowders prepared by coprecipitation and hydrothermal synthesis. J Eur Ceram Soc 27:921–926
Mody P et al (2016) Protein-based ferrogels. J Inorg Biochem 159:7–13
Morais P, Santos R, Pimenta A, Azevedo R, Lima E (2006) Preparation and characterization of ultra-stable biocompatible magnetic fluids using citrate-coated cobalt ferrite nanoparticles. Thin Solid Films 515:266–270
Mosaiab T et al (2013) Recyclable and stable silver deposited magnetic nanoparticles with poly (vinyl pyrrolidone)-catechol coated iron oxide for antimicrobial activity. Mater Sci Eng: C 33:3786–3794
Nadeem M et al (2016) Magnetic properties of polyvinyl alcohol and doxorubicine loaded iron oxide nanoparticles for anticancer drug delivery applications. PLoS ONE 11:e0158084
Namanga J, Foba J, Ndinteh DT, Yufanyi DM, Krause RWM (2013) Synthesis and magnetic properties of a superparamagnetic nanocomposite “pectin-magnetite nanocomposite”. J Nanomater 2013:1–8
Nikumbh A, Pawar R, Nighot D, Gugale G, Sangale M, Khanvilkar M, Nagawade A (2014) Structural, electrical, magnetic and dielectric properties of rare-earth substituted cobalt ferrites nanoparticles synthesized by the co-precipitation method. J Magn Magn Mater 355:201–209
Rajendran M, Pullar R, Bhattacharya A, Das D, Chintalapudi S, Majumdar C (2001) Magnetic properties of nanocrystalline CoFe2O4 powders prepared at room temperature: variation with crystallite size. J Magn Magn Mater 232:71–83
Rana S, Philip J, Raj B (2010) Micelle based synthesis of cobalt ferrite nanoparticles and its characterization using fourier transform infrared transmission spectrometry and thermogravimetry. Mater Chem Phys 124:264–269
Rouhani AR, Esmaeil-Khanian AH, Davar F, Hasani S (2018) The effect of agarose content on the morphology, phase evolution, and magnetic properties of CoFe2O4 nanoparticles prepared by sol-gel autocombustion method international. J Appl Ceram Technol 15:758–765
Salunkhe A, Khot V, Thorat N, Phadatare M, Sathish C, Dhawale D, Pawar S (2013) Polyvinyl alcohol functionalized cobalt ferrite nanoparticles for biomedical applications. Appl Surf Sci 264:598–604
Samaniego-Benitez JE, Ramírez-Aparicio J, Chavez-Urbiola I, Garcia-Garcia A, Perez-Robles JF, Ramirez-Bon R (2018) Synthesis and photocatalysis study of multiwalled carbon nanotubes grown in a lead-based microspherical support fullerenes. Nanotub Carbon Nanostruct 26:370–378
Sharifi I, Shokrollahi H, Doroodmand MM, Safi R (2012) Magnetic and structural studies on CoFe2O4 nanoparticles synthesized by co-precipitation, normal micelles and reverse micelles methods. J Magn Magn Mater 324:1854–1861
Singh RP, Ramarao P (2012) Cellular uptake, intracellular trafficking and cytotoxicity of silver nanoparticles. Toxicol Lett 213:249–259
Spizzo F et al (2017) Synthesis of ferrofluids made of iron oxide nanoflowers: interplay between carrier fluid and magnetic properties. Nanomaterials 7:373
Srinivasan SY, Paknikar KM, Bodas D, Gajbhiye V (2018) Applications of cobalt ferrite nanoparticles in biomedical nanotechnology. Nanomedicine 13:1221–1238
Srivastava V, Kohout T, Sillanpää M (2016) Potential of cobalt ferrite nanoparticles (CoFe2O4) for remediation of hexavalent chromium from synthetic and printing press wastewater. J Environ Chem Eng 4:2922–2932
Suharyadi E, Setiadi EA, Shabrina N, Kato T, Iwata S (2014) Magnetic properties and microstructures of polyethylene glycol (PEG)-coated cobalt ferrite (CoFe2O4) nanoparticles synthesized by coprecipitation method. Adv Mater Res 896:126–133
Tai MF, Lai CW, Abdul Hamid SB (2016) Facile synthesis polyethylene glycol coated magnetite nanoparticles for high colloidal stability. J Nanomater 2016:8612505. https://doi.org/10.1155/2016/8612505
Tatarchuk T et al (2017) Structural, optical, and magnetic properties of Zn-doped CoFe 2 O 4 nanoparticles. Nanoscale Res Lett 12:141
Vestal CR, Zhang ZJ (2002) Synthesis of CoCrFeO4 nanoparticles using microemulsion methods and size-dependent studies of their magnetic properties. Chem mater 14:3817–3822
Viltužnik B, Košak A, Zub YL, Lobnik A (2013) Removal of Pb (II) ions from aqueous systems using thiol-functionalized cobalt-ferrite magnetic nanoparticles. J Sol-Gel Sci Technol 68:365–373
Vinosha A, Jeronsia E, Raja K, Christina Fernandez A, Krishnan S, Das J (2016) Investigation of optical, electrical and magnetic properties of cobalt ferrite nanoparticles by naive co-precipitation technique. Optik 127:9917–9925
Volatron J et al (2017a) Ferritin protein regulates the degradation of iron oxide nanoparticles. Small 13:1602030
Volatron J et al (2017b) Physiological remediation of cobalt ferrite nanoparticles by ferritin. Sci Rep 7:40075
Wahab A, Imran M, Ikram M, Naz M, Aqeel M, Rafiq A, Majeed H, Ali S (2019) Dye degradation property of cobalt and manganese doped iron oxide nanoparticles. Appl Nanosci 9(8):1823–1832
Wang Z, Li Y, Viswan R, Hu B, Harris VG, Li J, Viehland D (2013) Engineered magnetic shape anisotropy in BiFeO3–CoFe2O4 self-assembled thin films. ACS Nano 7:3447–3456
Xiao SH, Jiang WF, Li LY, Li XJ (2007) Low-temperature auto-combustion synthesis and magnetic properties of cobalt ferrite nanopowder. Mater Chem Phys 106:82–87
Xue W et al (2018) Effects of core size and PEG coating layer of iron oxide nanoparticles on the distribution and metabolism in mice. Int J Nanomed 13:5719
Zhang H, Wang J, Zeng Y, Wang G, Han S, Yang Z, Li B, Wang X, Gao J, Zheng L, Liu X (2020) Leucine-coated cobalt ferrite nanoparticles: synthesis, characterization and potential biomedical applications for drug delivery. Phy Lett A 384(24):126600
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This work was financially supported by the Higher Education Commission Pakistan under NRPU project No. 6411.
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Akhtar, K., Javed, Y., Jamil, Y. et al. Functionalized cobalt ferrite cubes: toxicity, interactions and mineralization into ferritin proteins. Appl Nanosci 10, 3659–3674 (2020). https://doi.org/10.1007/s13204-020-01484-x
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DOI: https://doi.org/10.1007/s13204-020-01484-x