Abstract—

The effects of bright light and high temperature stresses on the genetic diversity of a Fragaria vesca L. clone recovered from meristems frozen in liquid nitrogen after two years of cultivation in vitro were analyzed. After two-year cultivation in vitro of post-cryogenic plant material, the percentage of polymorphic DNA-markers, which was half (4.5%) of its initial value after cryopreservation, immediately returned to its initial value (9%). The Shannon index increased insignificantly. The stress effects of high temperature or bright sunlight stress effects on plantlets of the post-cryogenic clone recovered after two years liquid nitrogen did not cause the appearance of additional polymorphic fragments or significant changes in the Shannon Index.

中文翻译：

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低温培养的草莓后无性系克隆的遗传多样性及非生物因素的影响

摘要—

分析了强光和高温胁迫对体外培养两年后从液氮中冷冻的分生组织中回收的草莓草莓无性系遗传多样性的影响。经过低温植物后体外培养两年后，多态性DNA标记的百分比（冷冻保存后为其初始值的一半（4.5％））立即恢复为初始值（9％）。香农指数微不足道。液氮在两年后恢复后对低温后恢复克隆的幼苗的高温或强光照射的胁迫作用不会引起其他多态性片段的出现或香农指数的显着变化。