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Genetic Diversity of a Post-Cryogenic Fragaria vesca L. Clone after Long-Term Cultivation in vitro and Influenced by Abiotic Factors

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Abstract—

The effects of bright light and high temperature stresses on the genetic diversity of a Fragaria vesca L. clone recovered from meristems frozen in liquid nitrogen after two years of cultivation in vitro were analyzed. After two-year cultivation in vitro of post-cryogenic plant material, the percentage of polymorphic DNA-markers, which was half (4.5%) of its initial value after cryopreservation, immediately returned to its initial value (9%). The Shannon index increased insignificantly. The stress effects of high temperature or bright sunlight stress effects on plantlets of the post-cryogenic clone recovered after two years liquid nitrogen did not cause the appearance of additional polymorphic fragments or significant changes in the Shannon Index.

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Funding

This work was supported by the Russian Foundation for Basic Research, project no. 14-04-31615.

COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology Russian Academy of Sciences, 127276, Moscow, Russia

    A. I. Solov’eva & O. N. Vysotskaya

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  1. A. I. Solov’eva
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  2. O. N. Vysotskaya
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Correspondence to A. I. Solov’eva.

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Translated by T. Borisova

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Solov’eva, A.I., Vysotskaya, O.N. Genetic Diversity of a Post-Cryogenic Fragaria vesca L. Clone after Long-Term Cultivation in vitro and Influenced by Abiotic Factors. Biol Bull Russ Acad Sci 47, 253–258 (2020). https://doi.org/10.1134/S1062359020030085

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