Abstract

The effect of plant antimicrobial peptides on the initiation of callus and embryonal suspensor masses, formation of somatic embryos, and germination of regenerants of Siberian larch has been studied. Protein/peptide extracts isolated from Amarantus retroflexus (seeds), Nigella sativa (seeds), and Elytrigia elongata (spikelets) have been used as objects of plant origin. Peptides have been introduced into the nutrient media at the stage of initiation of embryogenic cultures and somatic embryo germination. The stimulating effect of peptides on the formation of embryogenic cultures of Siberian larch has been found. No other differences in the dynamics of growth in the control and experimental regenerants have been observed. This study is supposed to contribute to enhancing the immunity of the clonal planting stock of Siberian larch.

中文翻译：

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植物抗菌肽在华北落叶松体外胚发生培养中的应用

摘要

已经研究了植物抗菌肽对愈伤组织和胚芽悬浮物的起始，体细胞胚的形成以及西伯利亚落叶松再生剂的萌发的影响。从分离的蛋白质/肽提取物Amarantus苋（种子），果黑种草（种子），和长穗偃麦草（小穗）已被用作植物起源的对象。在胚胎发生培养和体细胞胚萌发的阶段，已将肽引入营养培养基。已经发现肽对西伯利亚落叶松的胚发生培养物的形成具有刺激作用。在对照和实验再生物中，没有观察到其他生长动态的差异。这项研究被认为有助于增强西伯利亚落叶松无性繁殖种群的免疫力。