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Use of Plant Antimicrobial Peptides in in vitro Embryogenic Cultures of Larix sibirica

  • DEVELOPMENTAL BIOLOGY
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Abstract

The effect of plant antimicrobial peptides on the initiation of callus and embryonal suspensor masses, formation of somatic embryos, and germination of regenerants of Siberian larch has been studied. Protein/peptide extracts isolated from Amarantus retroflexus (seeds), Nigella sativa (seeds), and Elytrigia elongata (spikelets) have been used as objects of plant origin. Peptides have been introduced into the nutrient media at the stage of initiation of embryogenic cultures and somatic embryo germination. The stimulating effect of peptides on the formation of embryogenic cultures of Siberian larch has been found. No other differences in the dynamics of growth in the control and experimental regenerants have been observed. This study is supposed to contribute to enhancing the immunity of the clonal planting stock of Siberian larch.

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Funding

This work was conducted within the framework of a Budget Project of Sukachev Institute of Forests, Siberian Branch, Russian Academy of Sciences, Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences” (project no. 0356-2017-0741) and supported by the Russian Foundation for Basic Research, the Government of Krasnoyarsk region, and the Krasnoyarsk Regional Science Foundation within the framework of research project no. 16-44-240509 “The Development of Biotechnology for the Production of Embryogenic Cultures of Siberian Larch Resistant to Fungal Diseases and Pests with the Use of Protective Antimicrobial Peptides in vitro” and no. 18-44-243004 “In vitro Studies of the Effect of Biologically Active Peptides of Plant and Microbial Origin on the Growth and Development of Conifers in Early Ontogenesis.”

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Authors and Affiliations

  1. Sukachev Institute of Forests, Siberian Branch, Russian Academy of Sciences, Division of Federal Research Center “Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences”, 660036, Krasnoyarsk, Russia

    I. N. Tretyakova, M. E. Pak, A. S. Shuklina & A. P. Pahomova

  2. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    E. A. Rogozhin

  3. Gause Institute of New Antibiotics, 119021, Moscow, Russia

    E. A. Rogozhin & V. S. Sadykova

  4. Tyumen State University, 625003, Tyumen, Russia

    E. A. Rogozhin

  5. Siberian Federal University, 660041, Krasnoyarsk, Russia

    I. N. Tretyakova & I. A. Petukhova

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  1. I. N. Tretyakova
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  2. E. A. Rogozhin
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  3. M. E. Pak
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  4. I. A. Petukhova
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  5. A. S. Shuklina
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  6. A. P. Pahomova
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  7. V. S. Sadykova
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Correspondence to I. N. Tretyakova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Romanova

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Tretyakova, I.N., Rogozhin, E.A., Pak, M.E. et al. Use of Plant Antimicrobial Peptides in in vitro Embryogenic Cultures of Larix sibirica. Biol Bull Russ Acad Sci 47, 225–236 (2020). https://doi.org/10.1134/S1062359020030097

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  • DOI: https://doi.org/10.1134/S1062359020030097

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