A highly selective and stable amperometric biosensor for the determination of the hypoxanthine (Hx) molecule was designed in this study. For this purpose, the enzyme electrode was prepared by immobilizing the xanthine oxidase (XnOx) and uricase (U) enzymes to the surface obtained by electrochemical polymerization in the presence of polypyrrole-paratoluenesulfonate (PPy-pTS) on the platinum (Pt) surface. The determination limit for the Hx molecule of the prepared biosensor was determined as 5 × 10⁻⁶ M, and the linear working range was determined as 5 ×10⁻⁶–5 × 10⁻³ M. At the end of 27 measurements, the biosensor preserved 70% of the initial amperometric response. At the end of the fourth 8 days, the enzyme electrode was observed to maintain 26% of the initial amperometric response. The K_M value for Pt/PPy-pTS-XnOxU enzyme electrode system prepared by immobilizing XnOxU was found to be 0.05 mM, and V_max was 0.56 μA/min. The effects of the interventions in biological environments on the biosensor response were examined. Also, since this biosensor has the potential to be used for the determination of Hx in synthetic samples, it can find an important field of study in the biological and food industry.

In this study, a highly selective and stable amperometric biosensor for determination of hypoxanthine was developed. For this reason, polypyrrole-paratoluenesulfonate films have been prepared on the platinum electrode by electropolymerization of pyrrole which was carried out in the presence of paratoluenesulfonate.

在这项研究中设计了一种用于测定次黄嘌呤（Hx）分子的高度选择性和稳定的安培生物传感器。为此目的，通过将黄嘌呤氧化酶（XnOx）和尿酸酶（U）固定在铂（Pt）表面上的聚吡咯-对甲苯磺酸盐（PPy-pTS）的存在下通过电化学聚合获得的表面上来制备酶电极。所制备的生物传感器的Hx分子的测定限为5×10 ^-6  M，线性工作范围为5×10 ^-6 –5×10 ^-3 M.在27次测量结束时，生物传感器保留了初始安培响应的70％。在第4天的第8天结束时，观察到酶电极维持初始安培反应的26％。发现通过固定XnOxU制备的Pt / PPy-pTS-XnOxU酶电极系统的K _M值为0.05 mM，V _max为0.56μA / min。研究了在生物环境中的干预措施对生物传感器响应的影响。此外，由于这种生物传感器具有用于测定合成样品中Hx的潜力，因此可以找到生物和食品工业中的重要研究领域。

在这项研究中，开发了一种用于测定次黄嘌呤的高度选择性和稳定的安培生物传感器。因此，通过在对甲苯磺酸盐的存在下进行的吡咯的电聚合，在铂电极上制备了聚吡咯-对甲苯磺酸盐膜。