Tetranychidae spider mites are considered key citrus pests in some production areas, especially Tetranychus urticae Koch. Over the past decades, pesticide overuse seems to have promoted T. urticae population selection in citrus orchards. However, the microbiota has also been pointed out as a plausible explanation for population structure or plant host specialisation observed in several arthropod species. In this work, we have determined the incidence of Cardinium, Rickettsia, Spiroplasma and Wolbachia as representatives of major distorter bacteria genera in Aplonobia histricina (Berlese), Eutetranychus banksi (McGregor), Eutetranychus orientalis (Klein), Panonychus citri (McGregor), Tetranychus evansi Baker and Pritchard, Tetranychus turkestani Ugarov and Nikolskii, and T. urticae populations from Spanish citrus orchards. Only Wolbachia was detected by PCR. The multilocus alignment approach and phylogenetic inference indicated that all detected Wolbachia belong to supergroup B. The deep analysis of each 16S rDNA, ftsZ and wsp gene sequences allowed identifying several phylogenetically different Wolbachia sequences. It probably indicates the presence of several different races or strains, all of them belonging to supergroup B. The wsp sequence typing analysis unveiled the presence of the two already identified alleles (61 and 370) and allowed to contribute with five new alleles, supporting the presence of different but related B-races in the studied mite populations. The results are discussed and related to T. urticae population structure, previously observed in Spanish citrus orchards.

中文翻译：

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柑桔果园螨类中心菜，立克次体，螺旋体和沃尔巴氏菌的分子特征。

在某些产区，特别是四叶螨科（Tetranychus urticae Koch），四叶螨科蜘蛛螨被认为是主要的柑橘害虫。在过去的几十年中，农药的过度使用似乎促进了柑桔园中锥虫的种群选择。然而，微生物群也已被指出为在几种节肢动物物种中观察到的种群结构或植物宿主专化的合理解释。在这项工作中，我们确定了心形，立克次体，螺旋体和Wolbachia的发生率，它们是Aplonobia histricina（Berlese），Eutetranychus banki（McGregor），东方大戟（Eutetranychus Orientalis）（克莱因），柑橘三色堇（McGregor），Tetranychus evansi Baker和Pritchard，Tetranychus turkestani Ugarov和Nikolskii，以及来自西班牙柑桔园的T. urticae种群。通过PCR仅检测到Wolbachia。多基因座比对方法和系统发育推断表明，所有检测到的沃尔巴克氏菌均属于超群B。对每个16S rDNA，ftsZ和wsp基因序列的深入分析可以鉴定出几个系统发育不同的沃尔巴克氏菌。序列。这可能表明几个不同种族或毒株的存在，都属于超群B.将他们的WSP序列分型分析，推出了两个已经确定的等位基因（61和370）的存在，并允许五个新等位基因的贡献，支持研究的螨种群中存在不同但相关的B种族。对结果进行了讨论，并与以前在西班牙柑桔园中观察到的荨麻疹种群结构有关。