Abstract

One of the current problems in regenerative medicine is the search for new approaches for skin restoration. A promising area is the use of biocompatible materials. Such constructs can serve as the basis for biomedical products designed to replace damaged tissue or function as wound dressings. In this work, photopolymerized films based on silk fibroin and methacrylated gelatin (F-MG) were created. An in vitro study revealed that the use of films as a substrate for the cultivation of NIH 3T3 fibroblasts and HaCaT keratinocytes leads to a change in the kinetics of cell growth. According to the MTT assay, the fibroblasts’ proliferation rate was lower on photopolymerized films, while that for keratinocytes was higher compared to culture plastic. The effect of the obtained films on skin regeneration was investigated in vivo in a model of a full-thickness wound of mouse skin. The use of F-MG films as wound dressings contributed to faster wound healing and more complete recovery of the skin structure compared to control (use of gauze). Animals of the experimental group exhibited the formation of hair follicles and the reduction of the scar area after 28 days.

中文翻译：

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基于纤维蛋白和明胶的光固化膜可促进皮肤再生

摘要

再生医学当前的问题之一是寻找皮肤修复的新方法。一个有前途的领域是使用生物相容性材料。此类构建体可以用作生物医学产品的基础，该产品旨在替代受损的组织或用作伤口敷料。在这项工作中，创建了基于丝素蛋白和甲基丙烯酸明胶（F-MG）的光致聚合膜。一项体外研究表明，将膜用作培养NIH 3T3成纤维细胞和HaCaT角质形成细胞的基质会导致细胞生长动力学的变化。根据MTT分析，与培养塑料相比，光聚合膜上的成纤维细胞增殖速率较低，而角质形成细胞的增殖速率较高。在小鼠皮肤全层伤口模型中体内研究了获得的膜对皮肤再生的作用。与对照（使用纱布）相比，使用F-MG膜作为伤口敷料有助于更快的伤口愈合和皮肤结构更完全的恢复。实验组的动物在28天后表现出毛囊的形成和疤痕面积的减少。