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Photocurable Films Based on Fibroin and Gelatin for Skin Regeneration

  • ORIGINAL RESEARCH
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Abstract

One of the current problems in regenerative medicine is the search for new approaches for skin restoration. A promising area is the use of biocompatible materials. Such constructs can serve as the basis for biomedical products designed to replace damaged tissue or function as wound dressings. In this work, photopolymerized films based on silk fibroin and methacrylated gelatin (F-MG) were created. An in vitro study revealed that the use of films as a substrate for the cultivation of NIH 3T3 fibroblasts and HaCaT keratinocytes leads to a change in the kinetics of cell growth. According to the MTT assay, the fibroblasts’ proliferation rate was lower on photopolymerized films, while that for keratinocytes was higher compared to culture plastic. The effect of the obtained films on skin regeneration was investigated in vivo in a model of a full-thickness wound of mouse skin. The use of F-MG films as wound dressings contributed to faster wound healing and more complete recovery of the skin structure compared to control (use of gauze). Animals of the experimental group exhibited the formation of hair follicles and the reduction of the scar area after 28 days.

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Funding

This work was financially supported by the Russian Foundation for Basic Research: complex project no. 17-00-00359 (scientific projects: nos. 17-00-00356, 17-00-00357, and 17-00-00358) and grant no. 18-34-00875 using the equipment purchased as part of the Development Program of Moscow University until 2020 and the equipment of the Shared Access Center of Moscow State University.

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Authors and Affiliations

  1. Subdepartment of Bioengineering, Department of Biology, Moscow State University, 119234, Moscow, Russia

    M. S. Kotliarova, A. S. Soldatenko, A. M. Moysenovich & K. V. Shaitan

  2. Laboratory of Confocal Microscopy, Department of Biology, Moscow State University, 119234, Moscow, Russia

    A. Yu. Arkhipova & M. M. Moisenovich

  3. Moscow Regional Research and Clinical Institute (“MONIKI”), 129110, Moscow, Russia

    A. Yu. Arkhipova

  4. AO EFFERON, Skolkovo Innovation Center, 121205, Moscow, Russia

    I. V. Bessonov

  5. State Research Institute of Genetics and Selection of Industrial Microorganisms, National Research Center Kurchatov Institute, 117545, Moscow, Russia

    L. I. Davydova, V. G. Bogush & V. G. Debabov

  6. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, 115478, Moscow, Russia

    A. A. Shtil

Authors
  1. M. S. Kotliarova
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  2. A. S. Soldatenko
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  3. A. Yu. Arkhipova
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  4. I. V. Bessonov
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  5. L. I. Davydova
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  6. A. M. Moysenovich
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  7. V. G. Bogush
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  8. V. G. Debabov
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  9. A. A. Shtil
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  10. M. M. Moisenovich
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  11. K. V. Shaitan
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Corresponding author

Correspondence to M. S. Kotliarova.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. The experiments were carried out in compliance with the ethical standards for working with animals established by the Moscow State University Bioethics Commission.

Additional information

Translated by K. Lazarev

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Kotliarova, M.S., Soldatenko, A.S., Arkhipova, A.Y. et al. Photocurable Films Based on Fibroin and Gelatin for Skin Regeneration. Moscow Univ. Biol.Sci. Bull. 75, 20–25 (2020). https://doi.org/10.3103/S0096392520010046

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  • DOI: https://doi.org/10.3103/S0096392520010046

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