Chenopodium album (common name Bathua) is a widely adapted weed plant facing wide array of temperatures (5–45 °C) during growth and development in North India. Antioxidant defense was studied in C. album leaves under water deficit stress, heat stress, water deficit stress and heat stress combination and water deficit preconditioning followed by heat stress. C. album plants subjected to water deficit stress and heat stress combination showed higher decline in water relations and lesser recovery compared to either stresses alone. Highest H₂O₂ content, lipid peroxidation and protein damage were observed in plants experiencing water deficit stress and heat stress combination which was coupled with less induction in activities of SOD, CAT and all AsA-GSH cycles enzymes and decline in AsA and GSH pool compared to plants subjected to either stress alone. Water deficit preconditioned C. album plants maintained higher activities of antioxidant defense enzymes and metabolites such as SOD, CAT, POX, DHAR, GSH content and AsA/DHA and GSH/GSSG ratios compared to non-preconditioned plants under heat stress. This is the first holistic report on effect of water deficit stress and heat stress combination and water deficit preconditioning followed by heat stress on ROS, damage and antioxidant defense including enzymes and metabolites in C. album. Water deficit stress and heat stress combination was more detrimental in C. album than either of the stresses alone as decline in water relations and increase in oxidative stress and damage was coupled with a decline in antioxidant defense both in enzymes i.e. SOD, CAT and AsA-GSH cycles enzymes and metabolites i.e. AsA and GSH content. Water deficit preconditioning followed by recovery resulted in induction of co-ordinated antioxidant defense in terms of both enzyme activities and metabolites during subsequent heat stress in C. album. Enhanced CAT activity and higher redox pool played a major role in cross tolerance in water deficit preconditioned C. album plants under heat stress.

中文翻译：

---

与仅在藜（Bathua）中的任何一种胁迫相比，增强的氧化胁迫，破坏和不足的抗氧化剂防御作用导致水分亏缺胁迫和热胁迫组合的恢复不足。

Chenopodium album（俗称Bathua）是一种适应广泛的杂草植物，在印度北部的生长和发育期间面临各种各样的温度（5-45°C）。在缺水胁迫，热胁迫，缺水胁迫和热胁迫组合以及缺水预处理和随后的热胁迫下研究了C.专辑叶子的抗氧化防御。与单独的任一种胁迫相比，经受缺水胁迫和热胁迫组合的C.专辑植物显示出较高的水分关系下降和较少的恢复。最高H ₂ O ₂与水分胁迫胁迫相比，经历水分亏缺胁迫和热胁迫组合的植物中其含量，脂质过氧化作用和蛋白质损伤均被观察到，同时与SOD，CAT和所有AsA-GSH循环酶的活性相比，SOD，CAT和所有AsA-GSH循环酶的诱导较少，AsA和GSH池下降要么单独强调。与未预处理的植物在热胁迫下相比，缺水的预处理的白色念珠菌植物保持较高的抗氧化防御酶和代谢产物（例如SOD，CAT，POX，DHAR，GSH含量以及AsA / DHA和GSH / GSSG比率）的活性。这是有关水分亏缺胁迫和热胁迫组合以及水分亏缺预处理，随后热胁迫对ROS，伤害和抗氧化剂防御（包括酶和代谢产物）的影响的第一份整体报告。。水分亏缺应力和热应力在组合是更不利藜比单独的应力作为在水中的关系，在氧化应激和损伤的增加下降是加上两个在酶即SOD，CAT和ASA-在抗氧化防御的下降GSH循环酶和代谢物，即AsA和GSH含量。在随后的热胁迫下，水分亏缺的预处理和随后的恢复导致诱导的抗氧化防御，包括抗酶活性和代谢产物，都受到C. album胁迫的影响。CAT活性增强和较高的氧化还原池在热胁迫下水分亏缺预处理的C. album植物中的交叉耐受性中起主要作用。