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Enhanced oxidative stress, damage and inadequate antioxidant defense contributes towards insufficient recovery in water deficit stress and heat stress combination compared to either stresses alone in Chenopodium album (Bathua)

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Abstract

Chenopodium album (common name Bathua) is a widely adapted weed plant facing wide array of temperatures (5–45 °C) during growth and development in North India. Antioxidant defense was studied in C. album leaves under water deficit stress, heat stress, water deficit stress and heat stress combination and water deficit preconditioning followed by heat stress. C. album plants subjected to water deficit stress and heat stress combination showed higher decline in water relations and lesser recovery compared to either stresses alone. Highest H2O2 content, lipid peroxidation and protein damage were observed in plants experiencing water deficit stress and heat stress combination which was coupled with less induction in activities of SOD, CAT and all AsA-GSH cycles enzymes and decline in AsA and GSH pool compared to plants subjected to either stress alone. Water deficit preconditioned C. album plants maintained higher activities of antioxidant defense enzymes and metabolites such as SOD, CAT, POX, DHAR, GSH content and AsA/DHA and GSH/GSSG ratios compared to non-preconditioned plants under heat stress. This is the first holistic report on effect of water deficit stress and heat stress combination and water deficit preconditioning followed by heat stress on ROS, damage and antioxidant defense including enzymes and metabolites in C. album. Water deficit stress and heat stress combination was more detrimental in C. album than either of the stresses alone as decline in water relations and increase in oxidative stress and damage was coupled with a decline in antioxidant defense both in enzymes i.e. SOD, CAT and AsA-GSH cycles enzymes and metabolites i.e. AsA and GSH content. Water deficit preconditioning followed by recovery resulted in induction of co-ordinated antioxidant defense in terms of both enzyme activities and metabolites during subsequent heat stress in C. album. Enhanced CAT activity and higher redox pool played a major role in cross tolerance in water deficit preconditioned C. album plants under heat stress.

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Abbreviations

AsA:

Ascorbate

APX:

Ascorbate peroxidase

CAT:

Catalase

DHAR:

Dehydroascorbate reductase

GSH:

Reduced glutathione

GR:

Glutathione reductase

GSSG:

Oxidized glutathione

MDHAR:

Monodehydroascorbate reductase

POX:

Guaiacol peroxidase

PVP:

Polyvinylpolypyrrolidone

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgements

This research was supported by the financial grants of CSIR Emeritus Scientist Scheme awarded to Dr. (Mrs.) R. K. Chopra.

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  1. Vimal Kumar Semwal

    Present address: Africa Rice Center (AfricaRice), C/O IITA, PMB 5320, Oyo Road, Ibadan, Nigeria

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  1. Stress Physiology Laboratory, Water Technology Centre, Indian Agricultural Research Institute, New Delhi, 110012, India

    Vimal Kumar Semwal & Renu Khanna-Chopra

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Correspondence to Renu Khanna-Chopra.

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Semwal, V.K., Khanna-Chopra, R. Enhanced oxidative stress, damage and inadequate antioxidant defense contributes towards insufficient recovery in water deficit stress and heat stress combination compared to either stresses alone in Chenopodium album (Bathua). Physiol Mol Biol Plants 26, 1331–1339 (2020). https://doi.org/10.1007/s12298-020-00821-2

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