Bioleaching experiments combined with X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and scanning electron microscopy (SEM) were conducted to investigate three kinds of bornites from different regions leached by moderately thermophilic mixed bacteria of Leptospirillum ferriphilum YSK, Acidithiobacillus caldus D1 and Sulfobacillus thermosulfidooxidans ST. The results of bioleaching experiments showed that the leaching efficiency and the redox potential were significantly increased. The copper extraction efficiencies of three kinds of bornite maintained rapid growth until around the 12th day and no longer increased after the 18th reaching 83.7%, 96.5% and 86.6%, respectively. The XRD results of the leaching residue indicated that three kinds of bornites all produced jarosite in the late stage of leaching, and the leaching residues from of Daye Museum and Yunnan Geological Museum contained a mass of elemental sulfur. XPS analysis and scanning electron microscopy experiments showed that the surface of mineral particles was jarosite and the copper in the leaching residue was almost dissolved.

结合X射线光电子能谱（XPS），X射线衍射（XRD）和扫描电子显微镜（SEM）进行了生物浸出实验，研究了铁钩端螺旋体YSK，嗜酸性硫杆菌的中等嗜热混合细菌浸出的不同区域的三种斑岩岩。喜温D1和硫化杆菌thermosulfidooxidansST。生物浸出实验的结果表明，浸出效率和氧化还原电位显着提高。三种铜铁矿的铜提取效率一直保持快速增长，直到第12天左右，而18号以后不再提高，分别达到83.7％，96.5％和86.6％。浸出渣的X射线衍射结果表明，三种铁矿均在浸出后期产生黄钾铁矾，大冶博物馆和云南地质博物馆的浸出渣中含有大量的元素硫。XPS分析和扫描电子显微镜实验表明，矿物颗粒的表面为黄钾铁矾，浸出残渣中的铜几乎溶解。