Organosolv pretreatment with two ethanol concentrations (25% and 50%, v/v) was performed to improve enzymatic saccharification of poplar sawdust. It was found that lower ethanol concentration (25%, v/v) pretreatment resulted in a higher enzymatic digestibility of poplar (38.1%) due to its higher xylan removal and similar lignin removal ratios, compared to that pretreated with 50% (v/v) ethanol pretreatment (27.5%). However, the residual lignin still exhibited a strong inhibition on enzymatic hydrolysis of organosolv-pretreated poplar (OP). Bio-surfactant preparations including tea saponin (TS), TS crude extract, and tea seed waste were applied in enzymatic hydrolysis of OP, due to their potential ability of reducing enzyme non-productive binding on lignin. Their optimal loadings in enzymatic hydrolysis of OP were optimized, which indicated that adding 0.075 g/g glucan of TS improved the 72-h glucose yield of OP by 48.3%. Moreover, adding TS crude extract and tea seed waste exhibited the better performance than TS for improving enzymatic hydrolysis of OP. It was verified that the presence of protein in TS crude extract and tea seed waste accounted for the higher improvement. More importantly, the directly application of tea seed waste in enzymatic hydrolysis could achieve the similar improvement on enzymatic hydrolysis of OP, where chemosynthetic surfactant (PEG6000) was added. The residual enzyme activities in supernatant of enzymatic hydrolysis were also determined to reveal the changes on enzyme adsorption after adding surfactants. Generally, tea seed waste could be directly applied as an alternative to chemosynthetic surfactants to promote enzymatic hydrolysis of lignocelluloses.

使用两种乙醇浓度（25% 和 50%，v/v）进行有机溶剂预处理以改善杨木锯末的酶促糖化。结果表明，与用 50% (v/v) 预处理的相比，较低的乙醇浓度 (25%, v/v) 预处理导致杨树的酶消化率更高 (38.1%)，因为它具有更高的木聚糖去除率和相似的木质素去除率。 v) 乙醇预处理 (27.5%)。然而，残留的木质素仍然对有机溶剂预处理的杨树 (OP) 的酶促水解表现出强烈的抑制作用。生物表面活性剂制剂包括茶皂素 (TS)、TS 粗提物和茶籽废料，由于它们具有减少酶非生产性结合木质素的潜在能力，因此被用于 OP 的酶促水解。优化了它们在 OP 酶促水解中的最佳负载量，这表明添加 0.075 g/g TS 葡聚糖可使 OP 的 72 小时葡萄糖产率提高 48.3%。此外，添加TS粗提物和茶籽废料在改善OP的酶解方面表现出优于TS的性能。经证实，TS 粗提物和茶籽废料中蛋白质的存在占更高的改进。更重要的是，将茶籽废料直接应用于酶解中，加入化学合成表面活性剂（PEG6000）后，对OP的酶解也有类似的改进。还测定了酶解上清液中残留的酶活性，以揭示加入表面活性剂后酶吸附的变化。一般来说，