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Promoting enzymatic saccharification of organosolv-pretreated poplar sawdust by saponin-rich tea seed waste

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Abstract

Organosolv pretreatment with two ethanol concentrations (25% and 50%, v/v) was performed to improve enzymatic saccharification of poplar sawdust. It was found that lower ethanol concentration (25%, v/v) pretreatment resulted in a higher enzymatic digestibility of poplar (38.1%) due to its higher xylan removal and similar lignin removal ratios, compared to that pretreated with 50% (v/v) ethanol pretreatment (27.5%). However, the residual lignin still exhibited a strong inhibition on enzymatic hydrolysis of organosolv-pretreated poplar (OP). Bio-surfactant preparations including tea saponin (TS), TS crude extract, and tea seed waste were applied in enzymatic hydrolysis of OP, due to their potential ability of reducing enzyme non-productive binding on lignin. Their optimal loadings in enzymatic hydrolysis of OP were optimized, which indicated that adding 0.075 g/g glucan of TS improved the 72-h glucose yield of OP by 48.3%. Moreover, adding TS crude extract and tea seed waste exhibited the better performance than TS for improving enzymatic hydrolysis of OP. It was verified that the presence of protein in TS crude extract and tea seed waste accounted for the higher improvement. More importantly, the directly application of tea seed waste in enzymatic hydrolysis could achieve the similar improvement on enzymatic hydrolysis of OP, where chemosynthetic surfactant (PEG6000) was added. The residual enzyme activities in supernatant of enzymatic hydrolysis were also determined to reveal the changes on enzyme adsorption after adding surfactants. Generally, tea seed waste could be directly applied as an alternative to chemosynthetic surfactants to promote enzymatic hydrolysis of lignocelluloses.

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Acknowledgements

This study was supported by the National Natural Science Foundation (31971606), the Natural Science Foundation of Jiangsu Province for Outstanding Youth (BK20190091), and State Key Laboratory of Bio-Fibers and Eco-Textiles (Qingdao University, No. K2019-15). The authors thank the Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD) for supporting the work.

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Authors and Affiliations

  1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Chenhuan Lai, Chundong Yang, Ying Zhao, Yuan Jia, Liwei Chen & Qiang Yong

  2. State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao, 266000, People’s Republic of China

    Chenhuan Lai & Chengfeng Zhou

  3. Key Laboratory of Forestry Genetics and Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing, 210037, People’s Republic of China

    Qiang Yong

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  1. Chenhuan Lai
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  2. Chundong Yang
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  3. Ying Zhao
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  4. Yuan Jia
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  5. Liwei Chen
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  7. Qiang Yong
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Correspondence to Qiang Yong.

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Lai, C., Yang, C., Zhao, Y. et al. Promoting enzymatic saccharification of organosolv-pretreated poplar sawdust by saponin-rich tea seed waste. Bioprocess Biosyst Eng 43, 1999–2007 (2020). https://doi.org/10.1007/s00449-020-02388-4

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