Allitol is a kind of rare sugar alcohol with potential application value. An engineered strain, which simultaneously expressed d-psicose-3-epimerase (DPE), ribitol dehydrogenase (RDH), and formate dehydrogenase (FDH) three enzymes, was constructed by cloning above three genes into one plasmid and transformed into the host E. coli strain, and used as the whole-cell catalysts for biotransformation of allitol from the low-cost substrate of d-fructose. The whole cell allitol biotransformation conditions were optimized. The medium, recombinant gene induction conditions, and the substrate feeding rate for cultivation of the catalytic cells were optimized. Then, the fed-batch culture was made and scaled up to 10 L fermentor. Finally, 63.44 g/L allitol was obtained from 100 g/L d-fructose after 3 h of biotransformation, and the allitol crystals of 99.9% purity were obtained by using cooling recrystallization. The allitol production method developed in this research has high product purity, and is highly efficient, easily scaled up, and suitable for large-scale production of highly purified allitol.

Allitol是一种稀有的糖醇，具有潜在的应用价值。通过将上述三个基因克隆到一个质粒中，构建了一个工程菌株，该菌株同时表达d -psoseose-3-epimerase（DPE），核糖醇脱氢酶（RDH）和甲酸脱氢酶（FDH）三个酶，并将其转化为一个质粒并转化到宿主E中。大肠埃希菌菌株，并用作全细胞催化剂，从d的低成本底物中进行Allitol的生物转化-果糖。优化了全细胞阿糖醇的生物转化条件。优化了培养基，重组基因诱导条件和用于催化细胞培养的底物进料速率。然后，进行分批补料培养，并按比例放大至10 L发酵罐。最后，在生物转化3小时后，从100g / L d-果糖中获得63.44g / L的Allitol，并且通过使用冷却重结晶获得纯度为99.9％的Allitol晶体。本研究开发的Allitol生产方法具有较高的产品纯度，并且效率高，易于规模化，适合大规模生产高度纯化的Allitol。