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Efficient Allitol Bioproduction from d-Fructose Catalyzed by Recombinant E. coli Whole Cells, and the Condition Optimization, Product Purification

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Abstract

Allitol is a kind of rare sugar alcohol with potential application value. An engineered strain, which simultaneously expressed d-psicose-3-epimerase (DPE), ribitol dehydrogenase (RDH), and formate dehydrogenase (FDH) three enzymes, was constructed by cloning above three genes into one plasmid and transformed into the host E. coli strain, and used as the whole-cell catalysts for biotransformation of allitol from the low-cost substrate of d-fructose. The whole cell allitol biotransformation conditions were optimized. The medium, recombinant gene induction conditions, and the substrate feeding rate for cultivation of the catalytic cells were optimized. Then, the fed-batch culture was made and scaled up to 10 L fermentor. Finally, 63.44 g/L allitol was obtained from 100 g/L d-fructose after 3 h of biotransformation, and the allitol crystals of 99.9% purity were obtained by using cooling recrystallization. The allitol production method developed in this research has high product purity, and is highly efficient, easily scaled up, and suitable for large-scale production of highly purified allitol.

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Acknowledgments

The authors would like to thank Chengjia Zhang, Caiyun Sun from the Core Facilities for Life and Environmental Sciences, State Key Lab of Microbial Technology for help and guidance in the experiments.

Funding

This research was funded by the Key R & D Plan of Shandong Province in 2019 (2019GSF107015), and Shandong Province Science and Technology Development Project (2015GSF121016) of China.

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Authors and Affiliations

  1. State Key Lab of Microbial Technology, Shandong University (Qingdao), Qingdao, China

    Xin Wen, Chengjia Zhang, Xin Song, Jianqun Lin & Jianqiang Lin

  2. Shandong Academy of Chinese Medicine, Jinan, China

    Huibin Lin

  3. Qingdao Longding Biotech Limited Company, Qingdao, China

    Yilin Ren

  4. School of Biological Engineering, Qilu University of Technology, Jinan, China

    Can Li

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  1. Xin Wen
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Correspondence to Xin Song or Jianqiang Lin.

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Wen, X., Lin, H., Ren, Y. et al. Efficient Allitol Bioproduction from d-Fructose Catalyzed by Recombinant E. coli Whole Cells, and the Condition Optimization, Product Purification. Appl Biochem Biotechnol 192, 680–697 (2020). https://doi.org/10.1007/s12010-020-03359-x

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