当前位置: X-MOL 学术Chem. Commun. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex.
Chemical Communications ( IF 4.3 ) Pub Date : 2020-06-09 , DOI: 10.1039/d0cc02834g
Sisi Gao 1 , Ashish Radadiya 2 , Wenbo Li 3 , Huanting Liu 4 , Wen Zhu 5 , Valérie de Crécy-Lagard 6 , Nigel G J Richards 7 , James H Naismith 8
Affiliation  

The enzyme ForT catalyzes C–C bond formation between 5′-phosphoribosyl-1′-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5′-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.

中文翻译:


揭示 C-核苷生物合成中 CC 键形成的化学:C-糖苷合酶/PRPP 复合物的晶体结构。



ForT 酶催化 5′-磷酸核糖基-1′-焦磷酸 (PRPP) 和 4-氨基-1 H -吡唑-3,5-二羧酸酯之间形成 C-C 键,从而形成福霉素 A 5′ 生物合成中的关键中间体由卡尼哈拉链霉菌 (Streptomyces kaniharaensis)产生的-磷酸盐。我们报告了 ForT/PRPP 复合物的 2.5 Å 分辨率结构,并定位了对 PRPP 识别和催化至关重要的活性位点残基。
更新日期:2020-07-09
down
wechat
bug