Issue 55, 2020

Uncovering the chemistry of C–C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex

Abstract

The enzyme ForT catalyzes C–C bond formation between 5′-phosphoribosyl-1′-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5′-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.

Graphical abstract: Uncovering the chemistry of C–C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex

Supplementary files

Article information

Article type
Communication
Submitted
19 Apr 2020
Accepted
14 May 2020
First published
09 Jun 2020
This article is Open Access
Creative Commons BY license

Chem. Commun., 2020,56, 7617-7620

Uncovering the chemistry of C–C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex

S. Gao, A. Radadiya, W. Li, H. Liu, W. Zhu, V. de Crécy-Lagard, N. G. J. Richards and J. H. Naismith, Chem. Commun., 2020, 56, 7617 DOI: 10.1039/D0CC02834G

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