Fungal growth and development depend on adaptation to the particular pH of their environment. Ambient pH sensing implies the activation of the pacC signaling pathway, which then acts as a critical regulator for different physiological conditions. The PacC transcription factor may also be associated with the control of salt stress tolerance. In a pH-dependent manner, salinity stress is surpassed by changes in gene expression and coordinated activation of other signaling pathways, thus permitting survival in the challenging environment. In this study, we assessed the regulatory role of Trichophyton interdigitale PacC in response to pH variation and salinity stress. By employing gene expression analysis, we evaluated the influence of PacC in the modulation of salt stress-related genes, including the transcription factors crz1, egr2, and the MAP kinase hog1 in the dermatophyte T. interdigitale. In our analysis, we also included the evaluation of a potassium/sodium efflux P-type ATPase aiming to identify the role of PacC on its ion pumping activity. Here we demonstrated that salinity stress and buffered pH conditions might affect the pacC gene modulation in the dermatophyte T. interdigitale.

中文翻译：

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盐胁迫影响皮肤癣菌间指毛癣菌中转录因子 PacC 的 pH 依赖性调节

真菌的生长和发育取决于对其环境特定 pH 值的适应。环境 pH 感应意味着 pacC 信号通路的激活，然后作为不同生理条件的关键调节器。PacC 转录因子也可能与盐胁迫耐受性的控制有关。以 pH 依赖性方式，基因表达的变化和其他信号通路的协同激活超过了盐度胁迫，从而允许在具有挑战性的环境中生存。在这项研究中，我们评估了毛癣菌叉指 PacC 对 pH 变化和盐度胁迫的调节作用。通过基因表达分析，我们评估了 PacC 在调节盐胁迫相关基因中的影响，包括转录因子 crz1、egr2、和皮肤癣菌 T. interdigitale 中的 MAP 激酶 hog1。在我们的分析中，我们还包括对钾/钠流出 P 型 ATP 酶的评估，旨在确定 PacC 对其离子泵活性的作用。在这里，我们证明了盐分胁迫和缓冲 pH 条件可能会影响皮肤癣菌 T. interdigitale 中的 pacC 基因调节。