Microalgae have been widely considered as a promising alternative to renewable feedstock to biofuel production. Considering the many oleaginous strains of microalgae, this work assessed the potential of two species, Chlorella minutissima and Nannochloropsis gaditana, to be tested as raw material for ethyl esters production of biodiesel value. This work demonstrates an efficient pilot-size photoautotrophic growth of the two strains using 40-L bubble-column photobioreactors, providing lipid productivities of around 15.2 and 8.7 mg L⁻¹ day⁻¹ for C. minutissima and N. gaditana, respectively. The lipid-bearing biomass, which presented fatty acids composition similar to vegetable oils, such as soybean, rich in palmitic, oleic, and linoleic acids, was then assayed in direct trans/esterification reactions using ethanol as acyl donor, lipid extracting and solvent for the liquid phase in a pressurized reactor. The reactions, which were catalyzed by a heterogeneous acid catalyst (12-molybdophosphoric acid supported onto aluminum oxide), demonstrated an efficient route for producing a product mixture containing ester contents greater than 96.5 wt%, total conversion of triacylglycerols, and low levels of mono- and diacylglycerols, promoting an ethyl ester mixture with possible integration within the biodiesel market specifications.

微藻已被广泛认为是可再生原料用于生物燃料生产的有前途的替代品。考虑到许多微藻的产油菌株，这项工作评估了小球藻小球藻和小白菜小球藻两个物种的潜力，可以作为生产生物柴油的乙酯生产原料进行测试。这项工作证明了使用40 L气泡柱光生物反应器对这两个菌株进行有效的中试规模光合自养生长，从而为C. minutissima和N. gaditana提供了约15.2和8.7 mg L ^-1  day ^-1的脂质生产率。， 分别。然后，使用乙醇作为酰基供体，脂质提取和溶剂作为溶剂，在直接的转/酯化反应中测定了具有脂质的生物质，其脂肪酸组成类似于植物油，例如大豆，富含大豆，棕榈酸，油酸和亚油酸。加压反应器中的液相 通过多相酸催化剂（负载在氧化铝上的12-钼磷酸）催化的反应表明，生产包含酯含量大于96.5 wt％的产物混合物，三酰甘油的总转化率和低水平单酯的有效途径-和二酰基甘油，促进在生物柴油市场规范内可能整合的乙酯混合物。