The surface topology of biomaterial has a definite effect on the growth behavior of nerve cells for peripheral nerve regeneration. In this study, the silk fibroin (SF) film with different anisotropic microgroove/ridge was constructed by micropatterning technology. The effects of topologies width on the directional growth of dorsal root ganglion (DRG) neurons were evaluated. The results showed that the topological structure of the SF film with higher SF concentration was more clear and complete. The microtopography of the SF film with a concentration of 15% and a groove width of around 30 μm could effectively guide the directional growth of the nerve fibers of DRG. And nerve fibers could obviously form nerve fiber bundles which may have a certain pavement effect on the recovery of nerve function. The study indicated that the SF film with a specific width of the topological structure may have potential applications in the field of directional nerve regeneration.

生物材料的表面拓扑结构对周围神经再生的神经细胞的生长行为具有确定的影响。在这项研究中，通过微图案技术构建了具有不同各向异性微沟槽/脊的丝素蛋白（SF）薄膜。评估了拓扑宽度对背根神经节（DRG）神经元定向生长的影响。结果表明，SF浓度较高的SF薄膜的拓扑结构更清晰，完整。浓度为15％的SF膜的微观形貌和大约30μm的凹槽宽度可以有效地引导DRG神经纤维的定向生长。神经纤维明显形成神经纤维束，可能对神经功能的恢复有一定的作用。