The G2-S16 polyanionic carbosilane dendrimer is a promising microbicide that inhibits HSV-2 infection in vitro and in vivo in mice models. This G2-S16 dendrimer inhibits HSV-2 infection even in the presence of semen. Murine models, such as BALB/c female mice, are generally used to characterize host-pathogen interactions within the vaginal tract. However, the composition of endogenous vaginal flora remains largely undefined with modern microbiome analyses. It is important to note that the G2-S16 dendrimer does not change healthy mouse vaginal microbiome where Pseudomonas (10.2–79.1%) and Janthinobacterium (0.7–13%) are the more abundant genera. The HSV-2 vaginally infected female mice showed a significant microbiome alteration because an increase of Staphylococcus (up to 98.8%) and Escherichia (30.76%) levels were observed becoming these bacteria the predominant genera. BALB/c female mice vaginally-treated with the G2-S16 dendrimer and infected with the HSV-2 maintained a healthy vaginal microbiome similar to uninfected female mice. Summarizing, the G2-S16 polyanionic carbosilane dendrimer inhibits the HSV-2 infection in the presence of semen and prevents the alteration of mice female vaginal microbiome.

中文翻译：

---

阳离子树状聚合物G2-S16抑制单纯疱疹2型感染并保护小鼠阴道微生物组。

G2-S16聚阴离子碳硅烷树状聚合物是一种有前途的杀微生物剂，可在小鼠模型中体外和体内抑制HSV-2感染。即使在精液存在的情况下，这种G2-S16树状聚合物也可抑制HSV-2感染。鼠模型，例如BALB / c雌性小鼠，通常用于表征阴道内宿主-病原体的相互作用。然而，现代微生物组分析仍未明确内源性阴道菌群的组成。重要的是要注意，G2-S16树状大分子不会改变健康的小鼠阴道微生物组，其中假单胞菌（10.2–79.1％）和詹氏杆菌（0.7–13％）是更丰富的属。HSV-2阴道感染的雌性小鼠表现出明显的微生物组改变，因为葡萄球菌增加细菌（高达98.8％）和大肠埃希菌（30.76％）的水平已成为这些细菌的主要属。用G2-S16树状大分子进行阴道处理并感染HSV-2的BALB / c雌性小鼠，其健康的阴道微生物组与未感染的雌性小鼠相似。总而言之，G2-S16聚阴离子碳硅烷树状大分子在精液存在下抑制HSV-2感染，并防止小鼠雌性阴道微生物组发生改变。