Aim

The aim of the current study is to evaluate the prevalence of trichomoniasis in men and women in the north of Iran and to find genotypes in the positive clinical specimens based on T. vaginalis actin gene.

Materials and Methods

Women’s genital (n = 500) and men’s urine (n = 1500) samples were collected from the participants referred to clinics in Mazandaran Province, northern Iran, during 2006–2018. In addition, 1500 Pap smear specimens, archived in the Bu Ali Hospital, Sari City, Mazandaran Province, northern Iran, were examined. The specimens were examined based on parasitological methods, nested polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism, and phylogenetic analysis.

Results

Overall, 17 (0.48%) of 3500 specimens were positive by PCR. Total prevalence was 0.55% (n = 2000) for women, of which 500 (1.4%; n = 7) specimens were collected freshly, and 1500 (0.26%; n = 4) were Pap smears. Moreover, six (0.4%) out of 1500 men urine specimens were positive. Overall, genotypes G, E, and I were detected with the prevalence of seven (0.2%), seven (0.2%), and three (0.08%), respectively. There was no significant statistical difference among the prevalence of the detected genotypes (P > 0.05).

Conclusion

As a whole, the prevalence of trichomoniasis was low in the studied area in the north of Iran and, most importantly, the genotypes of E, G, and I were distributed among men and women in the province.

中文翻译：

---

伊朗北部阴道毛滴虫临床分离株的肌动蛋白基因分子分型。

目的

本研究的目的是评估伊朗北部男性和女性的滴虫病患病率，并在基于T的阳性临床标本中寻找基因型。阴道肌动蛋白基因。

材料和方法

2006 年 至 2018 年期间，从转诊到伊朗北部马赞达兰省诊所的参与者那里收集了女性生殖器 ( n  = 500) 和男性尿液 ( n = 1500) 样本。此外，还对伊朗北部马赞达兰省萨里市布阿里医院存档的 1500 份巴氏涂片标本进行了检查。根据寄生虫学方法、巢式聚合酶链反应 (PCR)、PCR 限制性片段长度多态性和系统发育分析对标本进行了检查。

结果

总体而言，3500 份样本中有 17 份（0.48%）通过 PCR 呈阳性。 女性的总患病率为 0.55% ( n = 2000)，其中 500 个 (1.4%；n  = 7) 样本是新鲜采集的，1500 个 (0.26%；n  = 4) 是巴氏涂片。此外，1500 份男性尿液样本中有 6 份（0.4%）呈阳性。总体而言，检测到基因型 G、E 和 I 的流行率分别为 7 (0.2%)、7 (0.2%) 和 3 (0.08%)。检测到的基因型的流行率之间没有显着的统计学差异（P  > 0.05）。

结论

总体而言，伊朗北部研究区滴虫病患病率较低，最重要的是，E、G、I基因型在该省男性和女性中均有分布。