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Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran

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Abstract

Aim

The aim of the current study is to evaluate the prevalence of trichomoniasis in men and women in the north of Iran and to find genotypes in the positive clinical specimens based on T. vaginalis actin gene.

Materials and Methods

Women’s genital (n = 500) and men’s urine (n = 1500) samples were collected from the participants referred to clinics in Mazandaran Province, northern Iran, during 2006–2018. In addition, 1500 Pap smear specimens, archived in the Bu Ali Hospital, Sari City, Mazandaran Province, northern Iran, were examined. The specimens were examined based on parasitological methods, nested polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism, and phylogenetic analysis.

Results

Overall, 17 (0.48%) of 3500 specimens were positive by PCR. Total prevalence was 0.55% (n = 2000) for women, of which 500 (1.4%; n = 7) specimens were collected freshly, and 1500 (0.26%; n = 4) were Pap smears. Moreover, six (0.4%) out of 1500 men urine specimens were positive. Overall, genotypes G, E, and I were detected with the prevalence of seven (0.2%), seven (0.2%), and three (0.08%), respectively. There was no significant statistical difference among the prevalence of the detected genotypes (P > 0.05).

Conclusion

As a whole, the prevalence of trichomoniasis was low in the studied area in the north of Iran and, most importantly, the genotypes of E, G, and I were distributed among men and women in the province.

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Acknowledgements

The vice chancellor of research at the Mazandaran University of Medical Sciences supported this study with a grant (Code: 6101).

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Authors and Affiliations

  1. Department of Parasitology, Molecular and Cell Biology Research Center, Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Mazandaran University of Medical Sciences, Sari, Iran

    Hajar Ziaei Hezarjaribi, Mahbobeh Taghavi, Bahman Rahimi Esboei & Mehran Gholami

  2. Student Research Committee, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Karan Hasanjani Saravi

  3. Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran

    Roghiyeh Faridnia

  4. Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgān, Iran

    Hamed Kalani

  5. Department of Microbiology, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

    Ahmad Mardani

  6. Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgān, Iran

    Ogholniaz Jorjani

  7. Diabetes Research Center, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran

    Zahra Hosseinikhah

  8. Department of Parasitology, Toxoplasmosis Research Center, Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Farah-Abad Road, P.O. Box: 48471-91971, Sari, Iran

    Mahdi Fakhar

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  1. Hajar Ziaei Hezarjaribi
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  2. Mahbobeh Taghavi
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  11. Mahdi Fakhar
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Correspondence to Mahdi Fakhar.

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Ziaei Hezarjaribi, H., Taghavi, M., Hasanjani Saravi, K. et al. Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran. Acta Parasit. 65, 859–864 (2020). https://doi.org/10.2478/s11686-020-00226-x

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  • DOI: https://doi.org/10.2478/s11686-020-00226-x

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