Hydrogen production from microalgae has attracted considerable attention due to its high energy content and as a renewable and environmentally friendly energy source. Various strains of microalgae have been reported to produce “biohydrogen”, but screening for new strains is still necessary to discover strains with higher hydrogen yields. A newly isolated hydrogen-producing green alga was screened and labeled Chlorella sp. KLSc59. The effect of extracellular pH, light intensity, external carbon sources, reducing agents, and nutrient deprivation on biohydrogen production of Chlorella sp. KLSc59 were investigated. Hydrogen yield was higher under anaerobic conditions. Under external pH 7.2 with 53.2 μmol photons m⁻² s⁻¹ light intensity and using acetate as a carbon source, the optimum hydrogen yield was 281 μmol H₂ mg⁻¹ Chl. Nutrient deprivation reduced the hydrogen yield. Several reducing agents were assessed, and 1 mM ethanol enhanced yield by 3 times for 850 μmol H₂ mg⁻¹ Chl, and 1 mM sodium dithionite increased yield by 2.7 times for 750 μmol H₂ mg⁻¹ Chl. Significantly, our new strain showed higher hydrogen yields ranging from 1.5 to 68 times compared with other microalgae. Thus, Chlorella sp. KLSc59 showed valuable potential for biohydrogen production.

由于微藻的氢能含量高，是一种可再生和环保的能源，因此引起了人们的极大关注。已经报道了各种微藻类产生“生物氢”的方法，但是仍然有必要筛选出新的藻类以发现产氢量更高的菌株。筛选了新分离的产氢绿藻并标记为小球藻。KLSc59。细胞外pH，光强度，外部碳源，还原剂和营养剥夺对小球藻生物氢产生的影响。对KLSc59进行了调查。在厌氧条件下，氢气产率更高。在外部pH 7.2下，具有53.2μmol光子m ^-2  s ^-1光强度和使用乙酸盐作为碳源，最佳氢产率为281μmolH ₂ mg ^-1 Chl。营养剥夺降低了氢气产量。几个还原剂进行了评估，和1mM乙醇通过用于850微摩尔H 3倍增强的产量₂毫克^-1叶绿素和1mM连二亚硫酸钠2.7倍为750ħ微摩尔增加的产量₂毫克^-1叶绿素。值得注意的是，与其他微藻相比，我们的新菌株显示出更高的氢产率，为1.5至68倍。因此，小球藻属。KLSc59具有生产生物氢的宝贵潜力。