In recent years, the consumption of polyphenols has been increasing, largely due to its beneficial effects on health. They are present in a wide variety of foods, but their extraction and characterization are complicated since they are mostly in complex matrices. For this reason, the use of selective, sensitive, and versatile analytical techniques such as liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) is necessary. In this review, the most relevant studies of the last years regarding the analysis of polyphenols in different matrices by comprehensive LC–MS/MS are discussed. Relevant steps such as extraction, sample purification, and chromatographic analysis methods are emphasized. In particular, the following methodological aspects are discussed: (a) the proper selection of the extraction technique, (b) the extraction and elution solvents, (c) the purification step, (d) the selection of both stationary and mobile phases for the chromatographic separation of compounds, and (e) the different conditions for mass spectrometry. Overall, this review presents the data from the most recent studies, in a comprehensive way, thus providing and simplifying the information of the great variety of works that exist in the literature on this wide topic.

中文翻译：

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使用液相色谱-串联质谱技术（LC-MS / MS）测定多酚：综述。

近年来，多酚的消费一直在增加，这主要是由于其对健康的有益影响。它们存在于各种各样的食品中，但是由于它们大多存在于复杂的基质中，因此其提取和表征非常复杂。因此，必须使用选择性，灵敏且通用的分析技术，例如液相色谱与串联质谱联用（LC-MS / MS）。在这篇综述中，讨论了最近几年有关通过综合LC-MS / MS分析不同基质中多酚的最相关研究。强调了相关步骤，例如提取，样品纯化和色谱分析方法。特别要讨论以下方法方面的问题：（a）正确选择提取技术，（b）萃取和洗脱溶剂，（c）纯化步骤，（d）选择固定相和流动相进行化合物色谱分离，以及（e）质谱的不同条件。总体而言，本综述以一种全面的方式提供了来自最新研究的数据，从而提供并简化了有关这一广泛主题的文献中存在的大量作品的信息。