Abstract

Cancer testis antigens SEMG1 and SEMG2 (semenogelins 1 and 2) are the main proteins of human seminal fluid and are also expressed in malignant neoplasms of various origins. The biological role of semenogelins in the reproductive process is well understood, but almost nothing is known about their functions in tumor cells. In the present study, we used Mia-Paca2 cell line with overexpression of SEMG1 and SEMG2a as a cell model of human pancreatic cancer. Mia-Paca2 cells transduced with the corresponding vector served as control. Using flow cytometry and dihydroethidium (DHE) fluorescent agent detecting predominantly superoxide anion, we have shown that overexpression of SEMG1 and SEMG2 increases the ROS level. In addition, both semenogelins increased the susceptibility of tumor cells to the widely used chemotherapeutic genotoxic drugs doxorubicin and cisplatin. These findings showed that oncosuppressive properties are possessed by SEMG1 and SEMG2 in the cell model of pancreatic cancer.

中文翻译：

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人胰腺癌Mia-Paca2细胞模型中SEMG1和SEMG2癌-睾丸抗原的抑癌特性。

摘要

睾丸癌抗原SEMG1和SEMG2（半透明胶质蛋白1和2）是人类精液的主要蛋白质，并在各种起源的恶性肿瘤中表达。精液明胶在生殖过程中的生物学作用已广为人知，但对其在肿瘤细胞中的功能几乎一无所知。在本研究中，我们使用具有超表达SEMG1和SEMG2a的Mia-Paca2细胞系作为人类胰腺癌的细胞模型。用相应载体转导的Mia-Paca2细胞用作对照。使用流式细胞仪和二氢乙二胺（DHE）荧光剂检测主要是超氧阴离子，我们已经表明SEMG1和SEMG2的过表达增加了ROS水平。此外，两种精明蛋白均增加了对广泛使用的化学治疗性遗传毒性药物阿霉素和顺铂对肿瘤细胞的敏感性。这些发现表明，在胰腺癌的细胞模型中，SEMG1和SEMG2具有抑癌作用。