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Oncosupressor Properties of SEMG1 and SEMG2 Cancer-Testicular Antigens in the Cell Model of Human Pancreatic Carcinoma Mia-Paca2

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Abstract

Cancer testis antigens SEMG1 and SEMG2 (semenogelins 1 and 2) are the main proteins of human seminal fluid and are also expressed in malignant neoplasms of various origins. The biological role of semenogelins in the reproductive process is well understood, but almost nothing is known about their functions in tumor cells. In the present study, we used Mia-Paca2 cell line with overexpression of SEMG1 and SEMG2a as a cell model of human pancreatic cancer. Mia-Paca2 cells transduced with the corresponding vector served as control. Using flow cytometry and dihydroethidium (DHE) fluorescent agent detecting predominantly superoxide anion, we have shown that overexpression of SEMG1 and SEMG2 increases the ROS level. In addition, both semenogelins increased the susceptibility of tumor cells to the widely used chemotherapeutic genotoxic drugs doxorubicin and cisplatin. These findings showed that oncosuppressive properties are possessed by SEMG1 and SEMG2 in the cell model of pancreatic cancer.

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Funding

This study was supported by the Russian Science Foundation, project no. 17-75-10205.

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Correspondence to O. Yu. Shuvalov.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by I. Fridlyanskaya

Abbreviations: ROS—reactive oxygen species, CTA—cancer testis antigens, 2DHE—dihydroethidium, SEMG1—semenogelin 1, SEMG2—semenogelin 2.

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Shuvalov, O.Y., Kizenko, A.I., Petukhov, A.V. et al. Oncosupressor Properties of SEMG1 and SEMG2 Cancer-Testicular Antigens in the Cell Model of Human Pancreatic Carcinoma Mia-Paca2. Cell Tiss. Biol. 14, 124–128 (2020). https://doi.org/10.1134/S1990519X20020108

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  • DOI: https://doi.org/10.1134/S1990519X20020108

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