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The Effect of Atrial Natriuretic Peptide on Reorganization of Actin Cytoskeleton and Migration of Human Mesenchymal Stem Cells
Cell and Tissue Biology Pub Date : 2020-04-13 , DOI: 10.1134/s1990519x20020091
A. V. Revittser , V. I. Chubinsky-Nadezhdin , Yu. A. Negulyaev

Abstract

Atrial natriuretic peptide (ANP) is involved in the regulation of many processes in the body and can affect mechano-dependent functions and the actin cytoskeleton in different cells. The effects of ANP in human endothelial cells are currently being intensely investigated; yet, its influence on the properties of mesenchymal stem cells (MSCs) has been little studied. MSC cultivation in the presence of different biologically active substances is of great importance for regenerative medicine, since these substances may affect the cell properties, improving the results of therapy. It has been previously shown that cultivation in the presence of ANP leads to changes in the migration potential of MSCs derived from rat perirenal fat. In this work, we have studied the effects of ANP on the actin structures and migration of bone marrow-derived MSCs from a 5- to 6-week-old human embryo (FetMSC line). We have demonstrated the expression of ANP-binding receptors (A- and C-type) in these cells. Furthermore, we have found that cultivation (for 24 h prior to the experiment) in the presence of low concentrations of ANP (10 nM) induces actin cytoskeleton assembly and slows the migration of FetMSCs (as estimated via wound healing assay). At a high concentration of ANP (1000 nM), no changes are observed in the cytoskeleton and cell migration compared to the control. Thus, cultivation in the presence of ANP may induce reorganization of the actin cytoskeleton and affect the migration potential of MSCs.


中文翻译:

心钠素对肌动蛋白细胞骨架重组和人间充质干细胞迁移的影响

摘要

心钠素(ANP)参与体内许多过程的调节,并可能影响不同细胞的机械依赖性功能和肌动蛋白细胞骨架。目前正在深入研究ANP在人内皮细胞中的作用。然而,其对间充质干细胞(MSCs)的性质的影响还很少研究。在具有不同生物活性物质的情况下进行MSC培养对于再生医学非常重要,因为这些物质可能会影响细胞特性,从而改善治疗效果。先前已经表明,在ANP存在下的培养导致源自大鼠肾周脂肪的MSC的迁移潜力的改变。在这项工作中 我们已经研究了ANP对5至6周龄人类胚胎(FetMSC系)的肌动蛋白结构和骨髓来源MSC迁移的影响。我们已经证明了在这些细胞中ANP结合受体(A型和C型)的表达。此外,我们发现在低浓度的ANP(10 nM)存在下培养(实验前24小时)会诱导肌动蛋白细胞骨架装配,并减慢FetMSC的迁移(通过伤口愈合试验估计)。与对照相比,在高浓度的ANP(1000 nM)下,未观察到细胞骨架和细胞迁移的变化。因此,在ANP存在下进行培养可能会诱导肌动蛋白细胞骨架的重组,并影响MSC的迁移潜力。我们已经证明了在这些细胞中ANP结合受体(A型和C型)的表达。此外,我们发现在低浓度的ANP(10 nM)存在下培养(实验前24小时)会诱导肌动蛋白细胞骨架装配,并减慢FetMSC的迁移(通过伤口愈合试验估计)。与对照相比,在高浓度的ANP(1000 nM)下,未观察到细胞骨架和细胞迁移的变化。因此,在ANP存在下进行培养可能会诱导肌动蛋白细胞骨架的重组,并影响MSC的迁移潜力。我们已经证明了在这些细胞中ANP结合受体(A型和C型)的表达。此外,我们发现在低浓度的ANP(10 nM)存在下培养(实验前24小时)会诱导肌动蛋白细胞骨架装配,并减慢FetMSC的迁移(通过伤口愈合试验估计)。与对照相比,在高浓度的ANP(1000 nM)下,未观察到细胞骨架和细胞迁移的变化。因此,在ANP存在下进行培养可能会诱导肌动蛋白细胞骨架的重组,并影响MSC的迁移潜力。我们发现在低浓度的ANP(10 nM)存在下培养(实验前24小时)会诱导肌动蛋白细胞骨架装配,并减慢FetMSC的迁移(通过伤口愈合试验估计)。与对照相比,在高浓度的ANP(1000 nM)下,未观察到细胞骨架和细胞迁移的变化。因此,在ANP存在下进行培养可能会诱导肌动蛋白细胞骨架的重组,并影响MSC的迁移潜力。我们发现在低浓度的ANP(10 nM)存在下培养(实验前24小时)会诱导肌动蛋白细胞骨架装配,并减慢FetMSC的迁移(通过伤口愈合试验估计)。与对照相比,在高浓度的ANP(1000 nM)下,未观察到细胞骨架和细胞迁移的变化。因此,在ANP存在下进行培养可能会诱导肌动蛋白细胞骨架的重组,并影响MSC的迁移潜力。
更新日期:2020-04-13
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