Abstract
Atrial natriuretic peptide (ANP) is involved in the regulation of many processes in the body and can affect mechano-dependent functions and the actin cytoskeleton in different cells. The effects of ANP in human endothelial cells are currently being intensely investigated; yet, its influence on the properties of mesenchymal stem cells (MSCs) has been little studied. MSC cultivation in the presence of different biologically active substances is of great importance for regenerative medicine, since these substances may affect the cell properties, improving the results of therapy. It has been previously shown that cultivation in the presence of ANP leads to changes in the migration potential of MSCs derived from rat perirenal fat. In this work, we have studied the effects of ANP on the actin structures and migration of bone marrow-derived MSCs from a 5- to 6-week-old human embryo (FetMSC line). We have demonstrated the expression of ANP-binding receptors (A- and C-type) in these cells. Furthermore, we have found that cultivation (for 24 h prior to the experiment) in the presence of low concentrations of ANP (10 nM) induces actin cytoskeleton assembly and slows the migration of FetMSCs (as estimated via wound healing assay). At a high concentration of ANP (1000 nM), no changes are observed in the cytoskeleton and cell migration compared to the control. Thus, cultivation in the presence of ANP may induce reorganization of the actin cytoskeleton and affect the migration potential of MSCs.
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ACKNOWLEDGMENTS
We are grateful to E.A. Morachevskaya (Institute of Cytology, Russian Academy of Sciences) for discussing the results and comments that were helpful in preparing the manuscript for publication.
Funding
This work was financially supported by the Russian Science Foundation, project no.18-15-00106.
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Translated by M. Romanova
Abbreviations: FI—fluorescence intensity, MSC—mesenchymal stem cell, ANP—atrial natriuretic peptide, NPRA and NPRC—A-type (NPRA) and C-type (NPRC) natriuretic peptide receptors.
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Revittser, A.V., Chubinsky-Nadezhdin, V.I. & Negulyaev, Y.A. The Effect of Atrial Natriuretic Peptide on Reorganization of Actin Cytoskeleton and Migration of Human Mesenchymal Stem Cells. Cell Tiss. Biol. 14, 154–159 (2020). https://doi.org/10.1134/S1990519X20020091
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DOI: https://doi.org/10.1134/S1990519X20020091