Abstract

Methylation of histone H3 Lys4 (meН3К4) and Lys9 (meН3К9), as well as DNA methylation (meDNA), was investigated in rat hippocampal and neocortex neurons in an original model of severe hypobaric hypoxia (SHH) and hypoxic postconditioning (PostC). It was shown that, in hippocampal field CA1, a day after SHH, the content of meH3K4 increased, while the level of meDNA decreased. Later, the amount of meH3K9 decreased and the meDNA content increased. PostC increased meH3K4, normalized the level of meH3K9, and decreased the level of meDNA in the hippocampal field CA1 of rats that had survived SHH. In the neocortex, significant changes were detected only 1–2 days after SHH, consisting in the stimulation of histone H3 methylation and decreased meDNA. Thus, a complex pattern of changes in the methylation of H3 histone and DNA was observed in both the hippocampus and neocortex in response to SHH. However, the protective effect of PostC was accompanied by the correction of only hippocampal reactions, while methylation in the neocortex returned to the initial level regardless of the PostC.

中文翻译：

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严重缺氧和缺氧后处理对大鼠脑中DNA和组蛋白H3甲基化的影响

摘要

在严重低压缺氧（SHH）和低氧后处理（PostC）的原始模型中，研究了大鼠海马和新皮层神经元中组蛋白H3 Lys4（meН3К4）和Lys9（meН3К9）的甲基化以及DNA甲基化（meDNA）。结果表明，在SHH后的第二天，在海马CA1区，meH3K4的含量增加，而meDNA的水平下降。后来，meH3K9的量减少，而meDNA含量增加。PostC增加了meH3K4的水平，使meH3K9的水平正常化，并降低了SHH存活大鼠海马区域CA1中的meDNA水平。在新皮层中，仅在SHH后1-2天检测到显着变化，包括刺激组蛋白H3甲基化和降低meDNA。从而，响应SHH，在海马和新皮层均观察到H3组蛋白和DNA甲基化甲基化变化的复杂模式。但是，PostC的保护作用仅伴随着海马反应的纠正，而新皮质中的甲基化则不管PostC都恢复到初始水平。