Abstract
Methylation of histone H3 Lys4 (meН3К4) and Lys9 (meН3К9), as well as DNA methylation (meDNA), was investigated in rat hippocampal and neocortex neurons in an original model of severe hypobaric hypoxia (SHH) and hypoxic postconditioning (PostC). It was shown that, in hippocampal field CA1, a day after SHH, the content of meH3K4 increased, while the level of meDNA decreased. Later, the amount of meH3K9 decreased and the meDNA content increased. PostC increased meH3K4, normalized the level of meH3K9, and decreased the level of meDNA in the hippocampal field CA1 of rats that had survived SHH. In the neocortex, significant changes were detected only 1–2 days after SHH, consisting in the stimulation of histone H3 methylation and decreased meDNA. Thus, a complex pattern of changes in the methylation of H3 histone and DNA was observed in both the hippocampus and neocortex in response to SHH. However, the protective effect of PostC was accompanied by the correction of only hippocampal reactions, while methylation in the neocortex returned to the initial level regardless of the PostC.
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ACKNOWLEDGMENTS
The work was carried out on animals provided by the IF Biological Collection of the Russian Academy of Sciences.
Funding
The study was supported by the “Program of Fundamental Scientific Research of State Academies for 2014–2020” (GP-14, section 65) and the Russian Foundation for Basic Research (projects no. 17-04-00624 and no. 17-04-01592).
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Statement on the welfare of animals. The experiments were performed in accordance with the requirements formulated in the Directives of the European Community (86/609/EEC) on the protection of animals used for experimental research. The experimental protocols were approved by the Commission on the Humane Treatment of Animals of the Pavlov Institute of Physiology, Russian Academy of Sciences.
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Translated by I. Fridlyanskaya
Abbreviations: SHH—severe hypobaric hypoxia, MHH—moderate hypobaric hypoxia, PostC—postconditioning, meDNA—DNA methylation, meH3K4 and meН3К9—methylation of histone H3 Lys4 and Lys9, respectively.
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Vetrovoy, O.V., Tyulkova, E.I., Stratilov, V.A. et al. The Pattern of DNA and Histone H3 Methylation in Rat Brain in Response to Severe Hypobaric Hypoxia and Hypoxic Postconditioning. Cell Tiss. Biol. 14, 36–42 (2020). https://doi.org/10.1134/S1990519X20010101
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DOI: https://doi.org/10.1134/S1990519X20010101