Abstract

The effect of X-ray radiation (0.1 Gy) on the expression of a number of genes and regulatory RNA (miRNA and long noncoding RNA) in human lymphocytes and T-lymphoblastic cells (Jurkat cells) was studied. One hour after cell irradiation with a low dose of radiation, lymphocytes displayed p53 expression and a decreased level of mature miR-27a and miR-181a having mRNA of gene p53 as target. These cells also had inhibited NFkB activity, which was found from reduced mRNA content of RhoAcdc42 and IL6 genes. By 4 h, their expression was normalized. Unlike in the case of normal cells, increased content of mRNA of NFkB gene (p65) and mRNA of its target IL6 gene was observed in Jurkat cells during this period. Repeated irradiation of cells with 5 Gy carried out after 4 h showed the radiation adaptive response (AO) according to the criterion of lymphocyte survival and its absence in Jurkat cells. The difference between groups of 5 Gy and 0.1 + 5 Gy in lymphocytes that survived after 20 h revealed common AO features (mRNA of p53 gene, NEAT1, miR-181a, miR-107). The results indicate the activation of various intracellular systems after the stress with low doses of radiation on lymphocytes and Jurkat cells. This approach can be used to optimize the efficacy of radiation therapy when preirradiation with a small dose of radiation increases the radioresistance of normal tissues surrounding a tumor.

中文翻译：

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低剂量电离辐射对正常和恶性人类细胞中基因和非编码RNA表达的影响

摘要

研究了X射线辐射（0.1 Gy）对人淋巴细胞和T淋巴细胞（Jurkat细胞）中许多基因和调节性RNA（miRNA和长非编码RNA）表达的影响。用低剂量的放射线照射细胞一小时后，淋巴细胞显示出p53表达，以基因p53的mRNA为靶标的成熟miR-27a和miR-181a水平降低。这些细胞也具有抑制的NFkB活性，这是由于RhoA cdc42和IL6基因的mRNA含量降低而发现的。到4小时，它们的表达被标准化。与正常细胞不同，NFkB基因（p65）的mRNA及其靶标IL6的mRNA含量增加在此期间，在Jurkat细胞中观察到该基因。4小时后，对5 Gy的细胞进行重复照射显示出辐射适应性反应（AO），这是根据淋巴细胞存活及其在Jurkat细胞中不存在的标准得出的。20小时后存活的淋巴细胞中5 Gy和0.1 + 5 Gy组之间的差异揭示了常见的AO特征（p53基因的mRNA ，NEAT1，miR-181a，miR-107）。结果表明，淋巴细胞和Jurkat细胞受到低剂量辐射后，各种细胞内系统均被激活。当使用小剂量的辐射进行预辐照会增加肿瘤周围正常组织的抗辐射能力时，可以使用这种方法来优化放射治疗的功效。