Abstract

The influence of synthetic water-soluble uncharged block copolymers on the multiplication of cells in a culture is studied. Their hydrophilic blocks are represented by linear poly(ethylene glycol) or branched polyglycerol, and hydrophobic blocks, by poly(propylene oxide) or polydimethylsiloxane. MCF-7/Adr cells are incubated with the copolymers in a serum-free culture medium for one hour, and their number after three days of cultivation without a copolymer is determined. The number of cells increases only under the action of polymers containing a poly(ethylene glycol) block, i.e., the longer this block, the lower the concentration of the polymer sufficient for the increase. The replacement of poly(ethylene glycol) by a hydrophilic block with a different structure, branched polyglycerol, fully cancels such an effect, thus indicating the defining role of poly(ethylene glycol) in the ability of block copolymers to maintain the multiplication of cells.

中文翻译：

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Pluronics提高亲水性聚环氧乙烷嵌段决定的细胞存活率的能力

摘要

研究了合成的水溶性不带电嵌段共聚物对培养物中细胞增殖的影响。它们的亲水嵌段以直链聚（乙二醇）或支链聚甘油为代表，疏水嵌段以聚环氧丙烷或聚二甲基硅氧烷为代表。将MCF-7 / Adr细胞与共聚物在无血清培养基中孵育一小时，并在无共聚物培养三天后测定其数目。孔数仅在含有聚乙二醇嵌段的聚合物的作用下增加，即，该嵌段越长，足以增加的聚合物浓度越低。用具有不同结构的亲水性嵌段（支链聚甘油）代替聚乙二醇可以完全消除这种影响，