A novel and uncomplicated synthesis method of Cu 2+ -chelating with carboxyl groups that directly-modified NiFe 2 O 4 magnetic microspheres (NiFe 2 O 4 -PAA-Cu 2+ ) was fabricated for selective enrichment and separation of bovine hemoglobin (BHb). First, a carboxyl group directly-modified on NiFe 2 O 4 magnetic microspheres was gained through a facile one-pot solvothermal method. Second, Cu 2+ from CuSO 4 was brought into use to react with carboxyl groups under mechanical stirring at room temperature. The resulting magnetic microspheres were characterized by distinct instruments that included transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM) and scanning electron microscope (SEM) to examine the size, morphology, composition and magnetization characterization. The results indicated that the NiFe 2 O 4 -PAA-Cu 2+ microspheres exhibited good saturation magnetization (36.686 emu g −1 ), which can facilitate magnetic separation under the help of an outside magnetic field. Also, good dispersion and high adsorption ability to BHb (783.53mg g −1 ) can be applied to selective enrichment for bovine hemoglobin and used for selective sorption of BHb protein in bovine blood samples.

中文翻译：

---

一种合成与铜 (II) 螯合的磁性纳米粒子以选择性吸附牛血红蛋白的简便方法

一种新型且简单的Cu 2+ -与羧基螯合的合成方法直接修饰NiFe 2 O 4 磁性微球（NiFe 2 O 4 -PAA-Cu 2+ ），用于选择性富集和分离牛血红蛋白（BHb） . 首先，通过简便的一锅溶剂热法在NiFe 2 O 4 磁性微球上直接修饰羧基。其次，利用CuSO 4 中的Cu 2+ 在室温下机械搅拌下与羧基反应。所得磁性微球通过不同的仪器进行表征，包括透射电子显微镜 (TEM)、Brunauer-Emmett-Teller (BET)、傅里叶变换红外光谱 (FT-IR)、X 射线光电子能谱 (XPS)、振动样品磁强计 ( VSM) 和扫描电子显微镜 (SEM) 来检查尺寸，形态、成分和磁化特性。结果表明，NiFe 2 O 4 -PAA-Cu 2+ 微球表现出良好的饱和磁化强度（36.686 emu g -1 ），可以在外磁场的帮助下促进磁分离。此外，良好的分散性和对BHb的高吸附能力（783.53mg g -1 ）可用于牛血红蛋白的选择性富集和牛血样中BHb蛋白的选择性吸附。