An insecticidal mannose binding lectin gene of Withania somnifera, WsMBP1 was constitutively expressed in tobacco plants. Instar-wise study on the response of Hyblaea puera larvae to the total protein extracted from transgenic tobacco was conducted and survivability percent was 33.33% and 55.55% in the first and the second instars, respectively. Minimum survivability of 22.22% was registered in the third instar. Further, two-fold reduction was observed in mean pre-pupal and pupal weight in the third instar larval populations fed with lectin protein compared to the control populations. The functional confirmation of the insecticidal activity of WsMBP1 established its potential as a novel gene resource for future transformation studies in developing teak genotypes tolerant to its leaf defoliator, H. puera.

杀虫甘露糖的凝集素的基因结合催眠睡茄，WsMBP1是组成在烟草植物中表达。进行了关于羽扇豆幼虫对从转基因烟草中提取的总蛋白的反应的龄期研究，第一龄和第二龄的存活率分别为33.33％和55.55％。第三龄鼠的最低生存率为22.22％。此外，喂食凝集素蛋白的三龄幼虫群体的平均pre前和up重量与对照群体相比降低了两倍。WsMBP1杀虫活性的功能确认确立了其作为新型基因资源的潜力，可用于开发可耐受其叶落叶者H. puera的柚木基因型的未来转化研究。