High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis.,Chemical Communications

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High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis.
Chemical Communications ( IF 4.3 ) Pub Date : 2020-06-01 , DOI: 10.1039/d0cc02844d
Fangzhi Yu ₁ , Zheng Yuan , Dapeng Zhang , Yan Liu , Qiang Zhao , Hailin Wang

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By identification of a super-stable protein–DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of hyper-recombinases generated from bioengineering and detection of single DNA mismatches caused by replication error.

中文翻译：

高亲和力和未分离的毛细管电泳，用于DNA链交换分析。

通过鉴定超稳定的蛋白质-DNA亲和力系统，我们开发了一种自由溶液毛细管电泳方法，可快速，灵敏地检测重组酶介导的根本重要的DNA链交换反应。我们进一步扩展了这种测定方法，以鉴定由生物工程产生的超重组酶和检测由复制错误引起的单个DNA错配。

更新日期：2020-07-07

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