Issue 54, 2020
From the journal:

Chemical Communications

High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis†

Fangzhi Yu, ORCID logo ab   Zheng Yuan, ORCID logo ab   Dapeng Zhang, ORCID logo ab   Yan Liu, ORCID logo ab   Qiang Zhao ORCID logo ab  and  Hailin Wang ORCID logo *abc  

* Corresponding authors

a State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, No. 18 Shuangqing Road, Beijing 100085, P. R. China
E-mail: hlwang@rcees.ac.cn

b University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, P. R. China

c Institute of Environment and Health, Jianghan University, No. 8, Sanjiaohu Road, Wuhan, Hubei, P. R. China

Abstract

By identification of a super-stable protein–DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of hyper-recombinases generated from bioengineering and detection of single DNA mismatches caused by replication error.

Graphical abstract: High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis

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Article information

DOI
https://doi.org/10.1039/D0CC02844D
Article type
Communication
Submitted
20 Apr 2020
Accepted
01 Jun 2020
First published
01 Jun 2020

Chem. Commun., 2020,56, 7403-7406

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High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis

F. Yu, Z. Yuan, D. Zhang, Y. Liu, Q. Zhao and H. Wang, Chem. Commun., 2020, 56, 7403 DOI: 10.1039/D0CC02844D

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