Biofilm-mediated multidrug resistance has turned into major challenge for the treatment of C. albicans infections. In the present study, actinomycetes (SS5) isolated from marine crustacean were investigated for their ability to inhibit C. albicans biofilm formation. Cultural, morphological and 16S rRNA analysis revealed that the isolated strain was Streptomyces diastaticus . Ethyl acetate bioactive fractions (6 µg mL −1 ) from SS5 showed potent antibiofilm activity against C. albicans . Light microscopic and CLSM analysis further substantiated the antibiofilm activity of the bioactive fraction against C. albicans. The bioactive fraction was subjected to FTIR and GC–MS for characterization. From GC–MS analysis, the presence of 31 compounds were revealed, among which the alkanes are predominantly present. Hence, further investigation for the potential of these bioactive compounds against C. albicans biofilm will help in the identification of promising candidate for the prevention of biofilm-mediated infection.

中文翻译：

---

从海洋甲壳动物 Portunus sanguinolentus 中分离出的 Streptomyces diastaticus 对白色念珠菌具有潜在的抗生物膜活性

生物膜介导的多药耐药性已成为治疗白色念珠菌感染的主要挑战。在本研究中，研究了从海洋甲壳类动物中分离出的放线菌 (SS5) 抑制白色念珠菌生物膜形成的能力。培养、形态学和 16S rRNA 分析表明，分离的菌株是 Streptomyces diastaticus。来自 SS5 的乙酸乙酯生物活性组分 (6 µg mL -1 ) 显示出针对白色念珠菌的有效抗生物膜活性。光学显微镜和 CLSM 分析进一步证实了生物活性部分对白色念珠菌的抗生物膜活性。生物活性部分经过 FTIR 和 GC-MS 进行表征。从 GC-MS 分析中，发现存在 31 种化合物，其中主要存在烷烃。因此，