Abstract
Biofilm-mediated multidrug resistance has turned into major challenge for the treatment of C. albicans infections. In the present study, actinomycetes (SS5) isolated from marine crustacean were investigated for their ability to inhibit C. albicans biofilm formation. Cultural, morphological and 16S rRNA analysis revealed that the isolated strain was Streptomyces diastaticus. Ethyl acetate bioactive fractions (6 µg mL−1) from SS5 showed potent antibiofilm activity against C. albicans. Light microscopic and CLSM analysis further substantiated the antibiofilm activity of the bioactive fraction against C. albicans. The bioactive fraction was subjected to FTIR and GC–MS for characterization. From GC–MS analysis, the presence of 31 compounds were revealed, among which the alkanes are predominantly present. Hence, further investigation for the potential of these bioactive compounds against C. albicans biofilm will help in the identification of promising candidate for the prevention of biofilm-mediated infection.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahsan T, Chen J, Zhao X, Irfan M, Wu Y (2017) Extraction and identification of bioactive compounds (eicosane and dibutyl phthalate) produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani AG-3 strain KX852461 to control target spot disease in tobacco leaf. AMB Express 7:54. https://doi.org/10.1186/s13568-017-0351-z
Augustine SK, Bhavsar SP, Kapadnis BP (2005) A non-polyene antifungal antibiotic from Streptomyces albidoflavus PU 23. J Biosciences 30:201–211. https://doi.org/10.1007/Bf02703700
Badiee P, Hashemizadeh Z (2014) Opportunistic invasive fungal infections: diagnosis & clinical management. Indian J Med Res 139:195–204
Chandra J, Ghannoum MA (2018) CD101, a Novel echinocandin, possesses potent antibiofilm activity against early and mature candida albicans biofilms. Antimicrob Agents Chemother. https://doi.org/10.1128/AAC.01750-17
Cui J, Ren B, Tong Y, Dai H, Zhang L (2015) Synergistic combinations of antifungals and anti-virulence agents to fight against Candida albicans. Virulence 6:362–371. https://doi.org/10.1080/21505594.2015.1039885
Eustaquio AS, Gust B, Galm U, Li SM, Chater KF, Heide L (2005) Heterologous expression of novobiocin and clorobiocin biosynthetic gene clusters. Appl Environ Microbiol 71:2452–2459. https://doi.org/10.1128/AEM.71.5.2452-2459.2005
Ezhilan BP, Neelamegam R (2012) GC-MS analysis of phytocomponents in the ethanol extract of Polygonum chinense. L Pharmacognosy Res 4:11–14. https://doi.org/10.4103/0974-8490.91028
Gulati M, Nobile CJ (2016) Candida albicans biofilms: development, regulation, and molecular mechanisms. Microbes Infect 18:310–321. https://doi.org/10.1016/j.micinf.2016.01.002
Guo L, Wu JZ, Han T, Cao T, Rahman K, Qin LP (2008) Chemical composition, antifungal and antitumor properties of ether extracts of Scapania verrucosa Heeg. and its endophytic fungus Chaetomium fusiforme. Molecules 13:2114–2125. https://doi.org/10.3390/molecules13092114
Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey's manual of determinative bacteriology. The Williams & Wilkins Co, Baltimore
Hopwood DA (2007) Therapeutic treasures from the deep. Nat Chem Biol 3:457–458. https://doi.org/10.1038/nchembio0807-457
Jabra-Rizk MA, Kong EF, Tsui C, Nguyen MH, Clancy CJ, Fidel PL Jr, Noverr M (2016) Candida albicans Pathogenesis: Fitting within the Host-Microbe Damage Response Framework. Infect Immun 84:2724–2739. https://doi.org/10.1128/IAI.00469-16
Kim DG et al (2012) Bahamaolides A and B, antifungal polyene polyol macrolides from the marine actinomycete Streptomyces sp. J Nat Prod 75:959–967. https://doi.org/10.1021/np3001915
Leetanasaksakul K, Thamchaipenet A (2018) Potential anti-biofilm producing marine actinomycetes isolated from sea sediments in Thailand. Agric Nat Resour 52:228–233
Madasu RHB, Muvva V, Munaganti RK, Dorigondla KR, Yenamandra V (2017) Bioactive-guided fractionation of diols from Streptomyces sp MSL. 3 Biotech. https://doi.org/10.1007/s13205-017-0649-1
Mohapatra B, Sar P, Kazy SK, Maiti MK, Satyanarayana T (2018) Taxonomy and physiology of Pseudoxanthomonas arseniciresistens sp. Nov., an arsenate and nitrate-reducing novel gammaproteobacterium from arsenic contaminated groundwater, India. PLoS ONE 13:e0193718. https://doi.org/10.1371/journal.pone.0193718
Nazemi M, Khoshkhoo Z, Motalebi A, Karim H (2010) Identification non polar component and antibacterial activities of Iophonla evistylus from Persian Gulf. Int J Environ Sci Te 6:92–197
Nithyanand P, Beema Shafreen RM, Muthamil S, Karutha Pandian S (2015) Usnic acid inhibits biofilm formation and virulent morphological traits of Candida albicans. Microbiol Res 179:20–28. https://doi.org/10.1016/j.micres.2015.06.009
Oksuz S, Sahin I, Yildirim M, Gulcan A, Yavuz T, Kaya D, Koc AN (2007) Phospholipase and proteinase activities in different Candida species isolated from anatomically distinct sites of healthy adults. Jpn J Infect Dis 60:280–283
Perlin DS (2015) Mechanisms of echinocandin antifungal drug resistance. Ann N Y Acad Sci 1354:1–11. https://doi.org/10.1111/nyas.12831
Perry DJ, Pasi KJ (1999) Hemostasis and thrombosis protocols Methods in molecular medicine. Humana Press, Totowa, p 31
Risdian C, Mozef T, Wink J (2019) Biosynthesis of Polyketides in Streptomyces. Microorganisms. https://doi.org/10.3390/microorganisms7050124
Shafreen RB, Seema S, Ahamed AP, Thajuddin N, Ali Alharbi S (2017) Inhibitory Effect of Biosynthesized Silver Nanoparticles from Extract of Nitzschia palea Against Curli-Mediated Biofilm of Escherichia coli. Appl Biochem Biotechnol 183:1351–1361. https://doi.org/10.1007/s12010-017-2503-7
Shafreen RM, Muthamil S, Pandian SK (2014) Inhibition of Candida albicans virulence factors by novel levofloxacin derivatives. Appl Microbiol Biotechnol 98:6775–6785. https://doi.org/10.1007/s00253-014-5719-2
Shaik M, Girija Sankar G, Iswarya M, Rajitha P (2017) Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10. J Genet Eng Biotechnol 15:87–94. https://doi.org/10.1016/j.jgeb.2017.02.004
Srivastava V, Dubey AK (2016) Anti-biofilm activity of the metabolites of Streptomyces chrestomyceticus strain ADP4 against Candida albicans. J Biosci Bioeng 122:434–440. https://doi.org/10.1016/j.jbiosc.2016.03.013
Subramani R, Sipkema D (2019) Marine Rare Actinomycetes: A Promising Source of Structurally Diverse and Unique Novel Natural Products. Mar Drugs. https://doi.org/10.3390/md17050249
Tsui C, Kong EF, Jabra-Rizk MA (2016) Pathogenesis of Candida albicans biofilm. Pathog Dis. https://doi.org/10.1093/femspd/ftw018
Watve MG, Tickoo R, Jog MM, Bhole BD (2001) How many antibiotics are produced by the genus Streptomyces? Arch Microbiol 176:386–390. https://doi.org/10.1007/s002030100345
Yapar N (2014) Epidemiology and risk factors for invasive candidiasis. Ther Clin Risk Manag 10:95–105. https://doi.org/10.2147/TCRM.S40160
Zhang H, Hua Y, Chen J, Li X, Bai X, Wang H (2018) Organism-derived phthalate derivatives as bioactive natural products. J Environ Sci Heal C 36:125–144. https://doi.org/10.1080/10590501.2018.1490512
Acknowledgements
The authors sincerely thank Dr. Marina Zemser, Professor, Institute of Standards, Medical Laboratory, Ministry of Health, Israel and Dr. N. Suganthy, Department of Nanoscience and Technology, Alagappa University for reviewing and vetting the manuscript for English language.
Funding
The authors acknowledge DST–SERB for supporting this work through a grant to RMBS (File No. SR YSS/2014/000127). RMBS also acknowledge RUSA 2.O [F. 24–51/2014-U, Policy (TN Multi-Gen), Dept of Edn, GOI].
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
No potential conflict of interest was reported by the authors.
Additional information
Communicated by Erko Stackebrandt.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Siddharthan, S., Rajamohamed, B.S. & Gopal, V. Streptomyces diastaticus isolated from the marine crustacean Portunus sanguinolentus with potential antibiofilm activity against Candida albicans. Arch Microbiol 202, 1977–1984 (2020). https://doi.org/10.1007/s00203-020-01918-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00203-020-01918-8