Proteomic analysis reveals the damaging role of low redox laccase from Yersinia enterocolitica strain 8081 in the midgut of Helicoverpa armigera,Biotechnology Letters

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Proteomic analysis reveals the damaging role of low redox laccase from Yersinia enterocolitica strain 8081 in the midgut of Helicoverpa armigera
Biotechnology Letters ( IF 2.0 ) Pub Date : 2020-05-29 , DOI: 10.1007/s10529-020-02925-x
Shruti Ahlawat ₁ , Deepti Singh _{1,

2} , Asha Yadav ₁ , Amarjeet Kumar Singh ₃ , Jugsharan Singh Virdi ₄ , Krishna Kant Sharma ₁

Affiliation

Earlier, we have found that the enteropathogenic Yersinia enterocolitica have evolved the survival mechanisms that regulate the expression of laccase-encoding genes in the gut. The present study aims to characterize the purified recombinant laccase from Y. enterocolitica strain 8081 biovar 1B and understand its effect on the midgut of cotton bollworm, Helicoverpa armigera (Hübner) larvae. The recombinant laccase protein showed high purity fold and low molecular mass (~ 43 kDa). H. armigera larvae fed with laccase protein showed a significant decrease in body weight and damage in the midgut. Further, transmission electron microscopy (TEM) studies revealed the negative effect of laccase protein on trachea, malpighian tubules, and villi of the insect. The proteome comparison between control and laccase-fed larvae of cotton bollworm showed significant expression of proteolytic enzymes, oxidoreductases, cytoskeletal proteins, ribosomal proteins; and proteins for citrate (TCA cycle) cycle, glycolysis, stress response, cell redox homeostasis, xenobiotic degradation, and insect defence. Moreover, it also resulted in the reduction of antioxidants, increased melanization (insect innate immune response), and enhanced free radical generation. All these data collectively suggest that H. armigera (Hübner) larvae can be used to study the effect of microbes and their metabolites on the host physiology, anatomy, and survival.

中文翻译：

蛋白质组学分析揭示小肠结肠炎耶尔森菌 8081 低氧化还原漆酶对棉铃虫中肠的破坏作用

早些时候，我们发现肠道致病性小肠结肠炎耶尔森氏菌已经进化出调节肠道中漆酶编码基因表达的生存机制。本研究旨在表征来自小肠结肠炎耶尔森氏菌菌株 8081 biovar 1B 的纯化重组漆酶，并了解其对棉铃虫 Helicoverpa armigera (Hübner) 幼虫中肠的影响。重组漆酶蛋白显示出高纯度倍数和低分子量 (~ 43 kDa)。用漆酶蛋白喂养的棉铃虫幼虫表现出体重显着下降和中肠损伤。此外，透射电子显微镜 (TEM) 研究揭示了漆酶蛋白对昆虫气管、马氏小管和绒毛的负面影响。对照和漆酶喂养的棉铃虫幼虫的蛋白质组比较显示蛋白水解酶、氧化还原酶、细胞骨架蛋白、核糖体蛋白的显着表达；柠檬酸（TCA 循环）循环、糖酵解、应激反应、细胞氧化还原稳态、异生物质降解和昆虫防御的蛋白质。此外，它还导致抗氧化剂减少、黑化增加（昆虫先天免疫反应）和自由基生成增加。所有这些数据共同表明，棉铃虫 (Hübner) 幼虫可用于研究微生物及其代谢物对宿主生理、解剖和存活的影响。细胞氧化还原稳态、异生物降解和昆虫防御。此外，它还导致抗氧化剂减少、黑化增加（昆虫先天免疫反应）和自由基生成增加。所有这些数据共同表明，棉铃虫 (Hübner) 幼虫可用于研究微生物及其代谢物对宿主生理、解剖和存活的影响。细胞氧化还原稳态、异生物降解和昆虫防御。此外，它还导致抗氧化剂减少、黑化增加（昆虫先天免疫反应）和自由基生成增加。所有这些数据共同表明，棉铃虫 (Hübner) 幼虫可用于研究微生物及其代谢物对宿主生理、解剖和存活的影响。

更新日期：2020-05-29

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